Göbel E, Riessner R, Pohl P
Z Naturforsch C Biosci. 1976 Nov-Dec;31(11-12):687-92.
Euglena gracilis Klebs (strain Z) was grown heterotrophically in the dark for 6 days. Subsequently, the cells were grown photoautotrophically under white fluorescent light with varying amounts of 3-(3,4-dichlorophenyl)-1,1 dimethyl urea (DCMU) (1.7X10(-9)--1.0X10(-5)mol/l) added to the nutrient medium. Under non-photosynthetic conditions (addition of 10(-6)--10(-5)mol DCMU/l), the cells still produced chloroplast structures and a considerable percentage of compounds typical for chloroplasts, such as galactolipids (40-50%), chlorophylls (40%), and polyunsaturated C16 and C18 fatty acids (16:2, 16:3, 16:4, 18:2, 18:3) (65%). The results indicate that under these conditions the above compounds are synthesized from carbon sources outside the chloroplasts.
纤细裸藻（菌株Z）在黑暗中异养培养6天。随后，将细胞在白色荧光灯下进行光自养培养，向营养培养基中添加不同量的3-(3,4-二氯苯基)-1,1-二甲基脲（DCMU）（1.7×10⁻⁹ - 1.0×10⁻⁵mol/L）。在非光合条件下（添加10⁻⁶ - 10⁻⁵mol DCMU/L），细胞仍能产生叶绿体结构以及相当比例的典型叶绿体化合物，如半乳糖脂（40 - 50%）、叶绿素（40%）和多不饱和C16和C18脂肪酸（16:2、16:3、16:4、18:2、18:3）（65%）。结果表明，在这些条件下，上述化合物是由叶绿体外部的碳源合成的。
