大肠杆菌K-12在缺铁培养基中生长时某些芳香族氨基酸生物合成酶的去阻遏作用
Derepression of certain aromatic amino acid biosynthetic enzymes of Escherichia coli K-12 by growth in Fe3+-deficient medium.
作者信息
McCray J W, Herrmann K M
出版信息
J Bacteriol. 1976 Feb;125(2):608-15. doi: 10.1128/jb.125.2.608-615.1976.
Abstract
3-Deoxy-arabino-heptulosonic acid 7-phosphate synthase, prephenate dehydratase, tryptophan synthase, and 2,3-dihydroxybenzoylserine synthase enzyme activities are derepressed in wild-type Escherichia coli K-12 cells grown on Fe3+-deficient medium. This derepression is reversed when FeSO4 is added to the growth medium. Addition of shikimic acid to the Fe3+-deficient growth medium caused repression of the first three enzyme activities but not of 2,3-dihydroxybenzoylserine synthase activity. Addition of 2,3-dihydroxybenzoic acid to the Fe3+-deficient growth medium has no effect on any of the above-mentioned enzyme activities. The Fe3+ deficiency-mediated derepression of 3-deoxyarabino-heptulosonic acid 7-phosphate synthase activity is due to an elevation of the tyrosine-sensitive isoenzyme; the phenylalanine-sensitive isoenzyme is not derepressed under these conditions.
摘要
在缺铁培养基上生长的野生型大肠杆菌K-12细胞中，3-脱氧阿拉伯庚酮糖酸7-磷酸合酶、预苯酸脱水酶、色氨酸合酶和2,3-二羟基苯甲酰丝氨酸合酶的酶活性被解除抑制。当向生长培养基中添加硫酸亚铁时，这种解除抑制的状态会逆转。向缺铁生长培养基中添加莽草酸会导致前三种酶活性受到抑制，但对2,3-二羟基苯甲酰丝氨酸合酶活性没有影响。向缺铁生长培养基中添加2,3-二羟基苯甲酸对上述任何一种酶活性都没有影响。缺铁介导的3-脱氧阿拉伯庚酮糖酸7-磷酸合酶活性的解除抑制是由于酪氨酸敏感同工酶的升高；在这些条件下，苯丙氨酸敏感同工酶没有被解除抑制。
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