铁，芳香族化合物生物合成所必需的元素。
Iron, an essential element for biosynthesis of aromatic compounds.
作者信息
McCandliss R J, Herrmann K M
出版信息
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4810-3. doi: 10.1073/pnas.75.10.4810.
Abstract
Homogeneous preparations of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase [7-phospho-2-keto-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate lyase (pyruvate phosphorylating), EC 4.1.2.15] isolated as the enzyme-phosphoenolpyruvate complex from Escherichia coli are shown by atomic absorption analysis to contain approximately one mole of iron per mole of native enzyme. No cobalt was found, in contrast to suggestions of earlier workers. Pure enzyme preparations show a unique absorption maximum around 350 nm with an epsilon value of about 3500 M-1cm-1. The 350-nm band as well as the enzyme activity is lost when the enzyme is denatured with guanidine-hydrochloride, or when phosphoenolpyruvate, the first substrate to bind to the enzyme, is totally removed from the enzyme by incubation with an excess of erythrose 4-phosphate, the second substrate to bind to the enzyme. The iron remains bound to the enzyme when phosphoenolpyruvate is removed from the enzyme-phosphoenolpyruvate complex.
摘要
从大肠杆菌中分离得到的作为酶 - 磷酸烯醇丙酮酸复合物的3 - 脱氧 - D - 阿拉伯庚酮糖酸 - 7 - 磷酸合酶[7 - 磷酸 - 2 - 酮 - 3 - 脱氧 - D - 阿拉伯庚酮酸 - D - 赤藓糖 - 4 - 磷酸裂解酶（丙酮酸磷酸化），EC 4.1.2.15]的均一制剂，通过原子吸收分析表明，每摩尔天然酶含有约一摩尔铁。与早期研究者的推测相反，未发现钴。纯酶制剂在约350 nm处显示出独特的最大吸收峰，ε值约为3500 M-1cm-1。当酶用盐酸胍变性时，或者当与酶结合的第一个底物磷酸烯醇丙酮酸通过与过量的与酶结合的第二个底物赤藓糖 - 4 - 磷酸孵育而从酶中完全去除时，350 nm的吸收带以及酶活性都会丧失。当磷酸烯醇丙酮酸从酶 - 磷酸烯醇丙酮酸复合物中去除时，铁仍与酶结合。
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本文引用的文献
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Control of aromatic biosynthesis: the multiplicity of 7-phospho-2-oxo-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating) in Escherichia coli W.芳香族生物合成的调控：大肠杆菌W中7-磷酸-2-氧代-3-脱氧-D-阿拉伯庚酮酸-D-赤藓糖-4-磷酸裂解酶（丙酮酸磷酸化）的多样性
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Purification and properties of the 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (phenylalanine sensitive) of Escherichia coli K12. II. Inhibition of activity of the enzyme with phenylalanine and functional group-specific reagents.大肠杆菌K12的3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶（苯丙氨酸敏感型）的纯化及性质。II. 苯丙氨酸和官能团特异性试剂对该酶活性的抑制作用。
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An improved preparative method for D-erythrose 4-phosphate.一种改进的D-赤藓糖-4-磷酸制备方法。
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The separation of three allosterically inhibitable 3-deoxy-D-arabino-heptulosonate 7-phosphate synthases from extracts of Neurospora crassa and the purification of the tyrosine inhibitable isoenzyme.从粗糙脉孢菌提取物中分离出三种可别构抑制的3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶，并对酪氨酸可抑制的同工酶进行纯化。
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