Yukawa N, Kohno T, Ishikawa E, Takahama K
Department of Legal Medicine, Medical College of Miyazaki, Japan.
Forensic Sci Int. 1992 Aug;55(2):161-72. doi: 10.1016/0379-0738(92)90121-c.
A sensitive sandwich enzyme immunoassay for gamma-seminoprotein (p30, prostate-specific antigen) is described for sex discrimination of blood and bloodstains. A polystyrene ball coated with rabbit anti-gamma-seminoprotein IgG was incubated with gamma-seminoprotein and, after washing, with affinity-purified rabbit anti-gamma-seminoprotein Fab'-horseradish peroxidase conjugate. Peroxidase activity bound to the polystyrene ball was assayed by fluorometry using 3-(4-hydroxyphenyl)propionic acid as hydrogen donor. The detection limit of gamma-seminoprotein was 0.15 pg per assay. Blood levels of gamma-seminoprotein, measured using 1-10 microliters of blood, were at least 3.3-fold higher in male adults than in female adults. The ratio of gamma-seminoprotein in terms of pg to hemoglobin in terms of mg was significantly higher in male adults than in female adults. Thus, the measurement of gamma-seminoprotein or both gamma-seminoprotein and hemoglobin was useful for the discrimination of blood and bloodstains of male and female adults, although with some limitations.
本文描述了一种用于γ-精浆蛋白（p30，前列腺特异性抗原）的灵敏夹心酶免疫测定法，用于血液和血迹的性别鉴别。将包被有兔抗γ-精浆蛋白IgG的聚苯乙烯球与γ-精浆蛋白孵育，洗涤后，再与亲和纯化的兔抗γ-精浆蛋白Fab'-辣根过氧化物酶结合物孵育。以3-(4-羟基苯基)丙酸作为氢供体，通过荧光法测定结合在聚苯乙烯球上的过氧化物酶活性。每次测定γ-精浆蛋白的检测限为0.15 pg。使用1-10微升血液测得的成年男性血液中γ-精浆蛋白水平至少是成年女性的3.3倍。成年男性每pgγ-精浆蛋白与每mg血红蛋白的比值显著高于成年女性。因此，γ-精浆蛋白或γ-精浆蛋白与血红蛋白两者的测定对于鉴别成年男性和女性的血液及血迹是有用的，尽管存在一些局限性。
