Takahama K
Department of Legal Medicine, Miyazaki Medical College, Japan.
Forensic Sci Int. 1996 Jun 28;80(1-2):63-9. doi: 10.1016/0379-0738(96)01928-7.
A highly sensitive sandwich enzyme immunoassay for organ-specific antigen is described for use in forensic practice. The sandwich enzyme immunoassays for specific antigens to the liver (LSA), the small intestine (sucrase-isomaltase), and the heart (cardiac troponin I) were developed. High levels of antigen could be detected to exist in forensic materials, and it is clearly possible to differentiate between samples from these stabbed organs and those originating from other stabbed organs. In addition, a sandwich enzyme immunoassay for prostate-specific antigen (gamma-seminoprotein, gamma-sm) was developed for sex discrimination of blood and bloodstains. The ratio of gamma-sm to hemoglobin was significantly higher in male adults than in female adults.
本文描述了一种用于法医实践的高灵敏度器官特异性抗原夹心酶免疫测定法。开发了针对肝脏特异性抗原（LSA）、小肠（蔗糖酶-异麦芽糖酶）和心脏（心肌肌钙蛋白I）的特异性抗原的夹心酶免疫测定法。可检测到法医材料中存在高水平的抗原，并且能够明确区分来自这些被刺伤器官的样本与来自其他被刺伤器官的样本。此外，还开发了一种用于前列腺特异性抗原（γ-精蛋白，γ-sm）的夹心酶免疫测定法，用于血液和血迹的性别鉴定。成年男性中γ-sm与血红蛋白的比率显著高于成年女性。
