带有钙-45的花粉管细胞核的放射自显影片。
Autoradiographs of pollen tube nuclei with calcium-45.
作者信息
STEFFENSEN D, BERGERON J A
出版信息
J Biophys Biochem Cytol. 1959 Dec;6(3):339-42. doi: 10.1083/jcb.6.3.339.
Autoradiography with Ca(45) has been used to obtain information about the relation between calcium and chromosomes. Labelled pollen from the Easter lily, Lilium longiflorum, was allowed to develop into pollen tubes between 5 and 6 cm. long in the styles of non-radioactive flowers. All of the nuclei, namely the tube nucleus and the two sperm nuclei, retain Ca(45) after this period of growth and development. Since the two sperm nuclei have formed during this interval by the mitotic division of the generative nucleus and growth of the tube has occurred under the influence of the tube nucleus, it is inferred that the calcium was bound in a stable nuclear component, the chromosomes.
利用钙 - 45放射自显影技术来获取有关钙与染色体之间关系的信息。将来自麝香百合（Lilium longiflorum）的标记花粉置于非放射性花朵的花柱中，使其发育成长度在5至6厘米之间的花粉管。在这段生长和发育时期过后，所有的细胞核，即管核和两个精核，都保留了钙 - 45。由于这两个精核是在此期间由生殖核的有丝分裂形成的，并且花粉管的生长是在管核的影响下发生的，因此可以推断钙与一种稳定的核成分——染色体相结合。
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