分离细胞核中的镁和钙。
Magnesium and calcium in isolated cell nuclei.
作者信息
NAORA H, NAORA H, MIRSKY A E, ALLFREY V G
出版信息
J Gen Physiol. 1961 Mar;44(4):713-42. doi: 10.1085/jgp.44.4.713.
Abstract
The calcium and magnesium contents of thymus nuclei have been determined and the nuclear sites of attachment of these two elements have been studied. The nuclei used for these purposes were isolated in non-aqueous media and in sucrose solutions. Non-aqueous nuclei contain 0.024 per cent calcium and 0.115 per cent magnesium. Calcium and magnesium are held at different sites. The greater part of the magnesium is bound to DNA, probably to its phosphate groups. Evidence is presented that the magnesium atoms combined with the phosphate groups of DNA are also attached to mononucleotides. There is reason to believe that those DNA-phosphate groups to which magnesium is bound, less than 1/10th of the total, are metabolically active, while those to which histones are attached seem to be inactive.
摘要
已测定胸腺细胞核中的钙和镁含量，并研究了这两种元素的核附着位点。用于这些目的的细胞核是在非水介质和蔗糖溶液中分离出来的。非水细胞核含有0.024%的钙和0.115%的镁。钙和镁位于不同的位点。大部分镁与DNA结合，可能是与DNA的磷酸基团结合。有证据表明，与DNA磷酸基团结合的镁原子也与单核苷酸相连。有理由相信，与镁结合的DNA磷酸基团（占总数的不到1/10）具有代谢活性，而与组蛋白结合的那些似乎没有活性。
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