Howe D K, Vodkin M H, Novak R J, Mitchell C J, McLaughlin G L
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907.
J Am Mosq Control Assoc. 1992 Sep;8(3):333-5.
We recently developed an assay using the polymerase chain reaction (PCR) for the specific detection of St. Louis encephalitis (SLE) virus RNA. This assay was tested in a blind study on 7 samples of pooled mosquitoes (50 mosquitoes/pool) which were also characterized for SLE virus by plaque assay in Vero cell culture. One sample was positive for the SLE virus as determined by both the PCR assay and a combination of the plaque assay and the indirect fluorescent antibody assay. The remaining 6 samples were negative for the presence of SLE virus as determined by both methods. These data indicate that this PCR assay can be used to monitor for the presence of SLE virus in pools of homogenized mosquitoes. This approach could provide early data on which to base disease control decisions.
我们最近开发了一种利用聚合酶链反应（PCR）来特异性检测圣路易斯脑炎（SLE）病毒RNA的检测方法。在一项盲法研究中，该检测方法对7份混合蚊虫样本（每份样本50只蚊虫）进行了测试，这些样本同时也通过在Vero细胞培养中进行蚀斑试验来鉴定是否含有SLE病毒。通过PCR检测以及蚀斑试验与间接荧光抗体试验相结合的方法确定，有1份样本的SLE病毒呈阳性。通过这两种方法确定，其余6份样本均未检测到SLE病毒。这些数据表明，这种PCR检测方法可用于监测匀浆蚊虫样本中SLE病毒的存在情况。这种方法可为疾病控制决策提供早期数据依据。
