Adames A J, Dutary B, Tejera H, Adames E, Galindo P
Facultad de Ciencias Naturales y Exactas, Universidad de Panamá.
Rev Med Panama. 1993 May;18(2):106-19.
The authors studied for two years the role of the chicks of aquatic birds in the arboviral cycles in coastal lagoons in central Panama in order to determine the relation between Culex (Melanoconion) ocossa and Mansonia (Mansonia) dyari mosquitoes in the transmission and dissemination of the viruses of Saint Louis Encephalitis (SLE) and Venezuelan Equine Encephalitis (VEE). Mosquitoes were captured every fifteen days on two consecutive nights to isolate the virus, using light traps (CDC) and baited traps. The attempts to isolate the virus were made using Vero cell cultures and the determination of antibodies was performed. The results of the serologic tests seem to indicate that four bird species: the ex (?) heron (Bubulcus ibis), the American heron (Casmerodius albus), the spoon-billed duck (Cochlearius cochlearius) and the needle crow (Anhinga anhinga) could function as intermediate hosts in the transmission cycle of SLE. Two species, the ibis (Endocimus albus) and the spoon-billed duck (Cochlearius cochlearius) could also be intermediate hosts of VEE in the coastal lagoons of Panama. The presence of antibodies in chicks could indicate an infection acquired recently, after their birth, in this area. The VEE virus was recovered from blood filled mosquitoes which had fed on a spoon-billed duck probably infected and exposed in a Trinidad #10 trap. No SLE virus was isolated. Other unknown viruses were isolated from mosquitoes selected for these studies, such as C. ocossa and M. dyari. The results obtained with these studies indicate the need for more studies utilizing new field techniques in order to establish a link between SLE and VEE, the vector mosquitoes and the aquatic birds in the coastal lagoons of the area under investigation.
作者对水鸟雏鸟在巴拿马中部沿海泻湖虫媒病毒传播周期中的作用进行了为期两年的研究，以确定奥氏库蚊（黑须库蚊亚属）和达氏曼蚊（曼蚊属）在圣路易斯脑炎（SLE）和委内瑞拉马脑炎（VEE）病毒传播与扩散中的关系。每隔十五天在连续两个晚上使用灯光诱捕器（疾控中心诱捕器）和诱饵诱捕器捕获蚊子以分离病毒。使用Vero细胞培养物进行病毒分离尝试并检测抗体。血清学检测结果似乎表明，四种鸟类：牛背鹭（？）（夜鹭）、美洲鹭（大白鹭）、琵嘴鸭（角叫鸭）和蛇鹈（美洲蛇鹈）可能在SLE传播周期中充当中间宿主。两种鸟类，即白鹮（美洲白鹮）和琵嘴鸭（角叫鸭）也可能是巴拿马沿海泻湖VEE的中间宿主。雏鸟体内抗体的存在可能表明它们出生后在该地区近期感染。在一个特立尼达10号诱捕器中，从吸食了一只可能已感染并暴露的琵嘴鸭血液的饱血蚊子中分离出了VEE病毒。未分离出SLE病毒。从为这些研究挑选的蚊子中分离出了其他未知病毒，如奥氏库蚊和达氏曼蚊。这些研究获得的结果表明，需要利用新的现场技术进行更多研究，以建立SLE和VEE、媒介蚊子以及所研究沿海泻湖中的水鸟之间的联系。
