McCullough M J, Radden B G
School of Dental Science, University of Melbourne, Victoria, Australia.
J Oral Pathol Med. 1992 Sep;21(8):367-9. doi: 10.1111/j.1600-0714.1992.tb01367.x.
The aim of this investigation was to assess the reproducibility of a study which reported that involucrin could be used as a diagnostic marker in oral lichenoid lesions. Twenty-eight formalin-fixed, paraffin-embedded specimens were examined. They included tissue from 13 patients with lichen planus, 11 with drug-related lichenoid reactions, one with dysplasia in a lichenoid lesion and three with normal oral mucosa. In each case the biopsy site was the buccal mucosa, PAS stained smears for fungi were negative and immunofluorescence results supported the diagnosis. An immunoperoxidase technique was used to demonstrate involucrin reactivity. In all sections there was consistent positive staining for involucrin in the superficial layers of the epithelium and all the basal layers were negative. In conclusion there were no demonstrable differences in the involucrin reactivity between the different lesions.
本研究的目的是评估一项研究的可重复性，该研究报告称外皮蛋白可作为口腔扁平苔藓样病变的诊断标志物。检查了28个福尔马林固定、石蜡包埋的标本。它们包括来自13例扁平苔藓患者、11例药物相关扁平苔藓样反应患者、1例扁平苔藓样病变发育异常患者和3例正常口腔黏膜患者的组织。在每种情况下，活检部位均为颊黏膜，真菌的PAS染色涂片为阴性，免疫荧光结果支持诊断。采用免疫过氧化物酶技术来显示外皮蛋白反应性。在所有切片中，上皮表层的外皮蛋白均呈一致的阳性染色，而所有基底层均为阴性。总之，不同病变之间在外皮蛋白反应性方面没有明显差异。
