Greenstein R J, Colucci R D, Ybanez M M, Zhang R L, McElhinney A J, Bauman W A
Mt. Sinai School of Medicine, New York, New York.
J Surg Res. 1992 Jul;53(1):12-6. doi: 10.1016/0022-4804(92)90005-k.
To determine whether cholecystokinin (CCK), a small intestinal hormone, may have autocrine or paracrine functions, gene regulation in the rat stomach and duodenum has been evaluated following cytotoxic injury. We quantified total RNA, CCK messenger RNA (mRNA), total protein, small and large forms of CCK peptides and gastrin. The stomach and the intestine respond differently. Following cytotoxic injury duodenal total RNA falls (1.5 +/- 0.1 vs 0.18 +/- 0.04 mg/g P less than or equal to 0.0001), and CCK mRNA content is depleted (260 +/- 23 vs 41 +/- 8 pg CCK mRNA/duodenum P less than or equal to 0.0001), yet there is a paradoxical increase in CCK mRNA concentration (176 +/- 20 vs 303 +/- 38 pg CCK mRNA/mg total RNA P less than or equal to 0.01). Increases occurred in both molecular species of CCK peptides evaluated: CCK8 (8 +/- 7 vs 26 +/- 2 pmole/g P less than or equal to 0.0001), large forms of CCK (42 +/- 4 vs 250 +/- 27 pmole/g P less than or equal to 0.0001). By contrast, in the stomach, only decreases were observed. These data identify sites of anatomical and biosynthetic upregulation during gastrointestinal repair. Changes are dependent upon the length of the period of recovery, differ between stomach and duodenum, and may be age related. Intestinal CCK may have para- and or autocrine roles in addition to its hormone function.
为了确定小肠激素胆囊收缩素（CCK）是否具有自分泌或旁分泌功能，在细胞毒性损伤后对大鼠胃和十二指肠中的基因调控进行了评估。我们对总RNA、CCK信使RNA（mRNA）、总蛋白、CCK肽的小分子和大分子形式以及胃泌素进行了定量。胃和小肠的反应不同。细胞毒性损伤后，十二指肠总RNA下降（1.5±0.1对0.18±0.04mg/g，P≤0.0001），CCK mRNA含量减少（260±23对41±8pg CCK mRNA/十二指肠，P≤0.0001），然而CCK mRNA浓度却出现矛盾性增加（176±20对303±38pg CCK mRNA/mg总RNA，P≤0.01）。所评估的CCK肽的两种分子形式均增加：CCK8（8±7对26±2pmol/g，P≤0.0001），CCK大分子形式（42±4对250±27pmol/g，P≤0.0001）。相比之下，在胃中仅观察到下降。这些数据确定了胃肠道修复过程中解剖学和生物合成上调的部位。变化取决于恢复时间的长短，胃和十二指肠之间存在差异，并且可能与年龄有关。肠道CCK除了具有激素功能外，可能还具有旁分泌和/或自分泌作用。
