Greenstein R J, Isola L, Gordon J
Veterans Administration Medical Center, Bronx, New York.
Am J Med Sci. 1990 Jan;299(1):32-7. doi: 10.1097/00000441-199001000-00008.
Cholecystokinin (CCK) gene expression has been compared in the brain and duodenum of control and 5 days fasted rats. To study transcription, CCK mRNA was quantified using a solution hybridization assay. Large and small molecular weight CCK peptides were separated using a sequential extraction process and subsequently quantified by radioimmunoassay. In the duodenum, a fall in weight was paralleled by a decrease in CCK mRNA and in the large forms of CCK peptides. Small molecular species of CCK peptides did not change. There was no change in weight, CCK transcriptional or translational products in the brain as a whole. These data indicate location-specific differential regulation of the products of CCK gene expression in the fasted rat.
对正常大鼠和禁食5天的大鼠的大脑和十二指肠中的胆囊收缩素（CCK）基因表达进行了比较。为了研究转录情况，使用溶液杂交测定法定量CCK mRNA。通过连续提取过程分离大分子量和小分子量的CCK肽，随后通过放射免疫测定法定量。在十二指肠中，重量下降的同时CCK mRNA和大形式的CCK肽减少。CCK肽的小分子种类没有变化。整个大脑的重量、CCK转录或翻译产物没有变化。这些数据表明在禁食大鼠中CCK基因表达产物存在位置特异性差异调节。
