Popov S F, Kurilov V Ia, Lagutin M P
Mikrobiol Zh (1978). 1992 Jul-Aug;54(4):3-7.
Studies were carried out on guinea pigs and albino rats, intranasally infected with P. pseudomallei C-141. The cells of bronchovesicular exudate were obtained from animals 1, 4 and 24 hours after infection. Electron microscopy was applied to study the process of interaction of the agent and alveolar macrophages. Bacteria were shown to form a capsule which permitted avoiding phagocytosis, when entering the host respiratory system. Microbes that failed to form a capsule were absorbed by macrophages and enclosed in a phagosome. Then some bacteria were destroyed by the lysosomal enzymes, the other synthesized a capsule, which protected them against the effect of phagolysosome content. There were also such microbes which escaped from a phagosome prior to fusion with lysosomes and parasitized in phagocytic cytoplasma forming a capsule there. By the end of the first 24 hours of observation the intact encapsulated microbe species were found to prevail in the host cells.
对经鼻感染类鼻疽杆菌C - 141的豚鼠和白化大鼠进行了研究。在感染后1小时、4小时和24小时从动物身上获取支气管肺泡渗出液细胞。应用电子显微镜研究病原体与肺泡巨噬细胞的相互作用过程。结果显示，细菌形成了一个荚膜，当进入宿主呼吸系统时，该荚膜可避免被吞噬。未形成荚膜的微生物被巨噬细胞吞噬并包裹在吞噬体中。然后，一些细菌被溶酶体酶破坏，另一些则合成了荚膜，从而保护它们免受吞噬溶酶体内容物的影响。也有一些微生物在与溶酶体融合之前从吞噬体中逃脱，并寄生在吞噬细胞质中，在那里形成荚膜。在观察的前24小时结束时，发现完整的被包膜微生物种类在宿主细胞中占主导地位。
