Turpaev K T, Goriunov A V
Mol Biol (Mosk). 1992 May-Jun;26(3):601-16.
Consensus sequences of transcription factors IRF, E12/E47, MBF1 and MyoD1 were represented as combination of the short oligonucleotide patterns ("enhansones"). The ad hoc computer program was employed for analysis of distribution and localization of these patterns on the sequences of interferons, immunoglobulins, metallothioneins and muscle-specific genes. The set of patterns was strictly specific for each gene family. In enhancer regions of almost all genes from mentioned families patterns were gathered in compact groups surrounding the operator sites. This empirical rule is true for genes where two conditions were fulfilled. (1) Operator site consists of tandem or inverted repeat of the short oligonucleotide motif, for instance GAAA-GAA in interferons or CCA-TGG in immunoglobulins. (2) Multiplication of the operator site leads to increase of enhancer activity because of cooperative interactions between transcriptions factors.
转录因子IRF、E12/E47、MBF1和MyoD1的共有序列表示为短寡核苷酸模式（“增强子元件”）的组合。使用专门编写的计算机程序来分析这些模式在干扰素、免疫球蛋白、金属硫蛋白和肌肉特异性基因序列上的分布和定位。该模式集对每个基因家族都具有严格的特异性。在上述家族几乎所有基因的增强子区域中，模式聚集在围绕操纵位点的紧密簇中。这条经验规则适用于满足两个条件的基因。（1）操纵位点由短寡核苷酸基序的串联或反向重复组成，例如干扰素中的GAAA - GAA或免疫球蛋白中的CCA - TGG。（2）由于转录因子之间的协同相互作用，操纵位点的倍增会导致增强子活性增加。
