[质粒DNA介导的致病性假单胞菌转化]
[Transformation of pathogenic pseudomonas by plasmid DNA].
作者信息
Abaev I V, Akimova L A, Shitov V T, Volozhantsev N V, Svetoch E A
出版信息
Mol Gen Mikrobiol Virusol. 1992 Mar-Apr(3-4):17-20.
PMID:1383699
Abstract
The possibility has been shown of the genetical transformation of Pseudomonas mallei strains by the purified DNA of the plasmids RSF1010, pES154, pBS222 and pBR325. The frequency of transformation varied from 1.2 x 10(1) to 2.0 x 10(2) depending on the plasmid DNA and transformation technique used in the experiments. Pseudomonas pseudomallei cells could not be transformed by the methods described in the paper.
摘要
已证实，用质粒RSF1010、pES154、pBS222和pBR325的纯化DNA可对鼻疽假单胞菌菌株进行遗传转化。根据实验中使用的质粒DNA和转化技术，转化频率在1.2×10⁻¹至2.0×10⁻²之间变化。本文所述方法无法转化类鼻疽假单胞菌细胞。
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