Abaev I V, Astashkin E I, Pachkunov D M, Stagis N I, Shitov V T, Svetoch E A
Mol Gen Mikrobiol Virusol. 1995 Jan-Mar(1):28-36.
Comparative analysis of recipient activity of Pseudomonas mallei, Pseudomonas pseudomallei, and Pseudomonas cepacia strains towards naturally occurring and recombinant plasmid replicons was carried out. Autonomic broad host range vector plasmids based on RSF1010(IncQ) and pSa(IncW) replicons as well as integrative vectors based on pSUP202(Co1E1) replicon have been constructed. The study has shown that naturally occurring plasmids RSF1010(IncQ), pSa(IncW), R15(IncN), and RP4(IncP) are being efficiently transferred and stably maintained in investigated Pseudomonas strains. However, recombinant plasmids with the mini-replicon pSa which are stable in Escherichia coli have shown segregative instability in Pseudomonas strains, whereas derivatives of plasmid RSF1010 demonstrated different stability depending on the type of insertion. Plasmid pSUP202 derivative integrative vector pSM525 is efficiently introduced and stably maintained in P. mallei C-5 strain. Two vector systems for genetic manipulations in P.mallei and P.pseudomallei cells have been developed.
对鼻疽假单胞菌、类鼻疽假单胞菌和洋葱伯克霍尔德菌菌株对天然存在的和重组质粒复制子的受体活性进行了比较分析。构建了基于RSF1010(IncQ)和pSa(IncW)复制子的自主广宿主范围载体质粒以及基于pSUP202(Co1E1)复制子的整合载体。研究表明，天然存在的质粒RSF1010(IncQ)、pSa(IncW)、R15(IncN)和RP4(IncP)在被研究的假单胞菌菌株中能有效转移并稳定维持。然而，在大肠杆菌中稳定的带有微型复制子pSa的重组质粒在假单胞菌菌株中表现出分离不稳定性，而质粒RSF1010的衍生物根据插入类型表现出不同的稳定性。质粒pSUP202衍生物整合载体pSM525能有效导入并稳定维持在鼻疽假单胞菌C-5菌株中。已开发出两种用于鼻疽假单胞菌和类鼻疽假单胞菌细胞基因操作的载体系统。
