Ruiz E F, Rabago V M, Lecona S U, Perez A B, Ma T H
Centro de Estudios Academicos sobre Contaminacion Ambiental, Universidad Autonoma de Queretaro, Mexico.
Mutat Res. 1992 Nov 1;270(1):45-51. doi: 10.1016/0027-5107(92)90100-g.
The Tradescantia-micronucleus (Trad-MCN) bioassay was used to determine the clastogenicity of wastewater samples collected from the Arena canal which contains effluent from the industrial district Benito Juarez of the city of Queretaro, Mexico. Fifteen wastewater samples which were collected, in most cases, at bi-weekly intervals beginning in September 1986 through February 1988, after a 3-fold dilution were used to treat Tradescantia plant cuttings. The clastogenicity expressed in terms of micronucleus frequencies of treated groups (30 h of treatment without recovery time) was significantly (0.01) higher than that of the tapwater control groups. The Trad-MCN bioassay was also used for in situ monitoring of air pollutants for the clastogenicity at 3 sites near the industrial and residential areas (Flores Magon, Conalep and Bellas Artes) of the city of Queretaro. Fourteen monitoring trips were made to each of the 3 sites at monthly intervals beginning in May 1988 through June 1990. Seasonal variation of micronucleus frequencies was exhibited with the peak clastogenicities shown in May and June 1988, June 1989 and April 1990 at the three sites. Micronucleus frequencies of all the exposed groups at the Conalep site, a predominantly industrial area, were markedly higher than that of the laboratory control groups throughout the 2-year period.
紫露草微核（Trad-MCN）生物测定法被用于测定从阿雷纳运河采集的废水样本的致断裂性，该运河含有墨西哥克雷塔罗市贝尼托·华雷斯工业区的废水。1986年9月至1988年2月期间，大多每两周采集一次共15个废水样本，经3倍稀释后用于处理紫露草植物插条。处理组（处理30小时且无恢复时间）的微核频率所表示的致断裂性显著（0.01）高于自来水对照组。紫露草微核生物测定法还用于对克雷塔罗市工业和居民区（弗洛雷斯·马贡、科纳莱普和贝拉艺术宫）附近3个地点的空气污染物进行原位监测，以检测其致断裂性。1988年5月至1990年6月期间，每月对这3个地点各进行14次监测。微核频率呈现出季节性变化，在1988年5月和6月、1989年6月以及1990年4月，这3个地点的致断裂性达到峰值。在整个两年期间，在主要为工业区的科纳莱普地点，所有暴露组的微核频率均明显高于实验室对照组。
