Kliesch U, Adler I D
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Säugetiergenetik, Neuherberg, Germany.
Mutat Res. 1992 Dec;283(4):249-53. doi: 10.1016/0165-7992(92)90056-n.
The clastogenic effect of the antischistosomal drug hycanthone methanesulfonate was studied with the micronucleus test in mouse bone marrow cells. Male and female (102/El x C3H/El)F1 mice were treated with single i.p. injections. Bone marrow was sampled 18, 24 and 30 h after treatment with 100 mg/kg. The highest micronucleus yield occurred at 24 h. The dose response for micronucleus induction at 24 h after treatment was non-linear for doses between 5 and 300 mg/kg. The lowest effective dose was 5 mg/kg for females and 10 mg/kg for males. The experiments revealed a significantly higher sensitivity of female mice for the induction of micronuclei in polychromatic erythrocytes by hycanthone methanesulfonate. This result supports the recommendation to use both sexes for quantitative assessment of genotoxicity in the micronucleus test.
用小鼠骨髓细胞微核试验研究了抗血吸虫药甲磺羟萘醌的致断裂效应。对雄性和雌性(102/El×C3H/El)F1小鼠进行单次腹腔注射。用100mg/kg处理后18、24和30小时采集骨髓。微核产量最高出现在24小时。处理后24小时，5至300mg/kg剂量的微核诱导剂量反应呈非线性。最低有效剂量对雌性为5mg/kg，对雄性为10mg/kg。实验表明，甲磺羟萘醌诱导雌性小鼠多染性红细胞微核的敏感性显著更高。这一结果支持了在微核试验中使用两性进行遗传毒性定量评估的建议。
