Niikawa S, Hara A, Ando T, Sakai N, Yamada H, Shimokawa K
Department of Neurosurgery, Gifu University School of Medicine.
Neurol Med Chir (Tokyo). 1992 Aug;32(9):653-8. doi: 10.2176/nmc.32.653.
Investigation of lectin cytochemical staining of inflammatory cells in human gliomas showed that Allomyrina dichotoma (Allo-A) cytochemistry can reliably distinguish inflammatory from neoplastic cells. Allo-A cytochemistry combined with silver colloid staining of argyrophilic nucleolar organizer regions (Ag-NORs) was performed in human gliomas. Inflammatory cells possessed usually one but sometimes two Ag-NORs and macrophages often possessed several Ag-NORs. The mean Ag-NOR number per nucleus of inflammatory cells ranged from 1.81 to 2.34, and that of neoplastic cells ranged from 2.57 to 3.53 and from 2.84 to 4.46 in low- and high-grade gliomas, respectively. The mean Ag-NOR number per nucleus of inflammatory cells was significantly smaller than that of neoplastic cells (p less than 0.001). Combined Allo-A cytochemical and silver colloid Ag-NOR staining can provide a reliable Ag-NOR number in human gliomas by distinguishing inflammatory cells.
对人类胶质瘤中炎性细胞的凝集素细胞化学染色研究表明，独角仙凝集素（Allo - A）细胞化学能够可靠地区分炎性细胞和肿瘤细胞。在人类胶质瘤中进行了Allo - A细胞化学与嗜银核仁组织区（Ag - NORs）银胶体染色相结合的实验。炎性细胞通常有一个，但有时有两个Ag - NORs，巨噬细胞常常有多个Ag - NORs。炎性细胞每个细胞核的平均Ag - NOR数在1.81至2.34之间，在低级别和高级别胶质瘤中，肿瘤细胞的平均Ag - NOR数分别在2.57至3.53之间以及2.84至4.46之间。炎性细胞每个细胞核的平均Ag - NOR数显著小于肿瘤细胞（p小于0.001）。通过区分炎性细胞，Allo - A细胞化学和银胶体Ag - NOR染色相结合能够在人类胶质瘤中提供可靠的Ag - NOR数。
