Kajiwara K, Nishizaki T, Orita T, Nakayama H, Aoki H, Ito H
Department of Neurosurgery, Yamaguchi University School of Medicine, Japan.
J Neurosurg. 1990 Jul;73(1):113-7. doi: 10.3171/jns.1990.73.1.0113.
A silver colloid staining technique for identifying nucleolar organizer region-associated proteins (Ag-NOR's) was applied to 51 human gliomas. These comprised 20 glioblastomas multiforme, 15 anaplastic astrocytomas, and 16 astrocytomas, in which the mean numbers of Ag-NOR's per cell (+/- standard error of the mean) were 2.51 +/- 0.12, 2.01 +/- 0.10, and 1.76 +/- 0.06, respectively. Significant differences among these were recognized, and the mean number of Ag-NOR's paralleled the degree of histopathological malignancy. In 16 cases, studies were performed of the number of Ag-NOR's and the S-phase fraction by in vitro labeling using antibromodeoxyuridine monoclonal antibody. A linear relationship was demonstrated between these two factors (r = 0.857, p less than 0.001), although some scatter was seen. In 32 adult patients, the correlation between the number of Ag-NOR's and the prognosis was estimated. The results demonstrated that the group containing patients with less than 1.80 Ag-NOR's per cell had a better prognosis than the group with 1.80 Ag-NOR's or more. Thus, the number of Ag-NOR's reflected the degree of histopathological malignancy, S-phase fraction, and prognosis. Silver colloid staining for Ag-NOR's is a simple, rapid, and reproducible method for estimating the proliferative potential of human gliomas without requiring a complicated technique.
一种用于鉴定核仁组织区相关蛋白（Ag-NOR）的银胶体染色技术应用于51例人类胶质瘤。其中包括20例多形性胶质母细胞瘤、15例间变性星形细胞瘤和16例星形细胞瘤，每细胞Ag-NOR的平均数（±平均数的标准误）分别为2.51±0.12、2.01±0.10和1.76±0.06。这些之间存在显著差异，且Ag-NOR的平均数与组织病理学恶性程度平行。在16例病例中，使用抗溴脱氧尿苷单克隆抗体通过体外标记对Ag-NOR的数量和S期分数进行了研究。尽管存在一些离散，但这两个因素之间呈现线性关系（r = 0.857，p<0.001）。在32例成年患者中，评估了Ag-NOR数量与预后之间的相关性。结果表明，每细胞Ag-NOR少于1.80的患者组比Ag-NOR为1.80或更多的患者组预后更好。因此，Ag-NOR的数量反映了组织病理学恶性程度、S期分数和预后。用于Ag-NOR的银胶体染色是一种简单、快速且可重复的方法，无需复杂技术即可估计人类胶质瘤的增殖潜力。
