Cheng K W
Department of Physiology, University of Manitoba, Winnipeg, Canada.
Neurosci Lett. 1992 Aug 3;142(1):77-80. doi: 10.1016/0304-3940(92)90624-g.
A protein of neurite outgrowth activity has been identified in porcine seminal plasma after ammonium sulfate precipitation and affinity chromatography on heparin-Sepharose. Upon SDS-PAGE, the polypeptide is shown to have a M(r) of 16,000-18,000. Biologically by induction of neuritic processes on neuroblastoma cells, and immunologically by cross-reaction with specific antisera, this seminal plasma protein differs from acidic fibroblast growth factor (aFGF), basic fibroblast growth factor (bFGF) and nerve growth factor (NGF). The neurite outgrowth activity is relatively stable at pH 3-7 and under denaturing conditions of 8 M urea and beta-mercaptoethanol, but is inactivated by treatment of trypsin. This appears to be a novel protein, enhancing morphological differentiation of neuroblastoma cells in culture.
通过硫酸铵沉淀和肝素 - 琼脂糖亲和层析，在猪精浆中鉴定出一种具有神经突生长活性的蛋白质。经十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）分析，该多肽的相对分子质量（M(r)）为16,000 - 18,000。在生物学上，通过诱导神经母细胞瘤细胞产生神经突，以及在免疫学上，通过与特异性抗血清的交叉反应表明，这种精浆蛋白不同于酸性成纤维细胞生长因子（aFGF）、碱性成纤维细胞生长因子（bFGF）和神经生长因子（NGF）。该神经突生长活性在pH 3 - 7以及8 M尿素和β - 巯基乙醇的变性条件下相对稳定，但经胰蛋白酶处理后会失活。这似乎是一种新型蛋白质，可增强培养的神经母细胞瘤细胞的形态分化。
