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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Abdulla E M, Calaminici M, Campbell I C

Wellcome Research Laboratories, Beckenham, UK.

Clin Exp Pharmacol Physiol. 1995 May;22(5):362-3. doi: 10.1111/j.1440-1681.1995.tb02017.x.

The objectives of the study were to establish that inhibition of neuronal differentiation in culture (assessed by neurite outgrowth) can be used as a broad spectrum in vitro measure of neurotoxicity. 2. To establish whether a rapid measure of neurite outgrowth could be used. Thus the study examined the relationship between the degree of neurite outgrowth assessed directly by image analysis and neurofilament protein subunit levels measured by an ELISA. 3. SKNSH neuroblastoma cells, exposed for up to 6 days to mercuric chloride during initiation and continuation of differentiation, had lower levels of neurofilament proteins than unexposed cells. 4. Preliminary data from parallel examinations of neurite outgrowth assessed by image analysis and neurofilament protein subunit levels assessed by ELISA support a correlation when neurofilament protein levels are decreased by sub-cytotoxic doses of mercuric chloride in SKNSH cells.

本研究的目的是确定在培养中对神经元分化的抑制作用（通过神经突生长评估）可作为一种广谱的体外神经毒性测量方法。2. 确定是否可以使用一种快速的神经突生长测量方法。因此，该研究检测了通过图像分析直接评估的神经突生长程度与通过酶联免疫吸附测定法测量的神经丝蛋白亚基水平之间的关系。3. 在分化起始和持续过程中，将SKNSH神经母细胞瘤细胞暴露于氯化汞中长达6天，其神经丝蛋白水平低于未暴露的细胞。4. 通过图像分析评估神经突生长和通过酶联免疫吸附测定法评估神经丝蛋白亚基水平的平行检测的初步数据支持，当亚细胞毒性剂量的氯化汞使SKNSH细胞中的神经丝蛋白水平降低时，二者存在相关性。

Abdulla E M, Calaminici M, Campbell I C

Wellcome Research Laboratories, Beckenham, UK.

Clin Exp Pharmacol Physiol. 1995 May;22(5):362-3. doi: 10.1111/j.1440-1681.1995.tb02017.x.

The objectives of the study were to establish that inhibition of neuronal differentiation in culture (assessed by neurite outgrowth) can be used as a broad spectrum in vitro measure of neurotoxicity. 2. To establish whether a rapid measure of neurite outgrowth could be used. Thus the study examined the relationship between the degree of neurite outgrowth assessed directly by image analysis and neurofilament protein subunit levels measured by an ELISA. 3. SKNSH neuroblastoma cells, exposed for up to 6 days to mercuric chloride during initiation and continuation of differentiation, had lower levels of neurofilament proteins than unexposed cells. 4. Preliminary data from parallel examinations of neurite outgrowth assessed by image analysis and neurofilament protein subunit levels assessed by ELISA support a correlation when neurofilament protein levels are decreased by sub-cytotoxic doses of mercuric chloride in SKNSH cells.

本研究的目的是确定在培养中对神经元分化的抑制作用（通过神经突生长评估）可作为一种广谱的体外神经毒性测量方法。2. 确定是否可以使用一种快速的神经突生长测量方法。因此，该研究检测了通过图像分析直接评估的神经突生长程度与通过酶联免疫吸附测定法测量的神经丝蛋白亚基水平之间的关系。3. 在分化起始和持续过程中，将SKNSH神经母细胞瘤细胞暴露于氯化汞中长达6天，其神经丝蛋白水平低于未暴露的细胞。4. 通过图像分析评估神经突生长和通过酶联免疫吸附测定法评估神经丝蛋白亚基水平的平行检测的初步数据支持，当亚细胞毒性剂量的氯化汞使SKNSH细胞中的神经丝蛋白水平降低时，二者存在相关性。