Wilting J, Christ B, Weich H A
Anatomisches Institut II, Albert-Ludwigs-Universität, Freiburg, Federal Republic of Germany.
Anat Embryol (Berl). 1992 Aug;186(3):251-7. doi: 10.1007/BF00174147.
The in vivo effects of two growth factors, VEGF165 and PDGF-BB, were studied in the chick chorioallantoic membrane (CAM). The factors were air-dried on Thermanox discs and the inverted discs were placed on the day-13 CAM for a period of 3 days. The specimens were then fixed, examined under a stereomicroscope and processed for semi- and ultrathin sectioning. VEGF165 induces marked vascular growth. Many new blood vessels emerge from the precapillary arterioles, and a brush-like formation of vessels can be seen in this region. In the venous part of the vascular system, the formation of sinusoidal or lacunar vessels can be seen. Edema does not develop. PDGF-BB induces thickening of the CAM due to extracellular-matrix production and the proliferation or immigration of fibrocytes. These lie just beneath the ectodermal epithelium and are oriented parallel to it. Out of the four factors we have already studied (PDGF-BB, VEGF165, Angiogenin, bFGF), only VEGF165 specifically induces the growth of blood vessels.
在鸡胚绒毛尿囊膜（CAM）中研究了两种生长因子VEGF165和PDGF - BB的体内效应。将这些因子风干在Thermanox载玻片上，然后将倒置的载玻片放置在第13天的CAM上3天。然后将标本固定，在体视显微镜下检查，并进行半薄和超薄切片处理。VEGF165可诱导显著的血管生长。许多新血管从毛细血管前小动脉中出现，并且在该区域可以看到血管呈刷状形成。在血管系统的静脉部分，可以看到窦状或腔隙状血管的形成。未出现水肿。PDGF - BB由于细胞外基质的产生以及纤维细胞的增殖或迁移而导致CAM增厚。这些细胞位于外胚层上皮下方，并与之平行排列。在我们已经研究过的四种因子（PDGF - BB、VEGF165、血管生成素、碱性成纤维细胞生长因子）中，只有VEGF165能特异性诱导血管生长。
