Wilting J, Christ B, Bokeloh M, Weich H A
Anatomisches Institut II, Albert-Ludwigs-Universität, Freiburg, Germany.
Cell Tissue Res. 1993 Oct;274(1):163-72. doi: 10.1007/BF00327997.
The effect of vascular endothelial growth factor (VEGF165) on the chorioallantoic membrane (CAM) of 13-day-old chick embryos was studied. The factor was applied in doses of 0.5-4 micrograms for a period of up to 4 days. Macroscopical, histological and immunohistological studies were carried out. The localization of the factor was examined with an anti-VEGF antibody. The mitogenicity of VEGF165 and basic fibroblast growth factor (bFGF) were studied by means of the BrdU-anti-BrdU method. Furthermore, the effect of heparin alone and in combination with VEGF165 was investigated. VEGF165 specifically induces angiogenesis in doses of 0.5 microgram and more. A brush-like formation of blood vessels can be seen in the region of the precapillary vessels. Angiogenesis also takes place in the region of the capillaries and the venules. Histologically we found indications of sprouting as well as of intussusceptive capillary growth. The presence of the factor in the application area could be demonstrated with the anti-VEGF antibody for a period of 3 days. The factor is located in the chorionic epithelium and the intraepithelial capillaries. The BrdU-studies show that VEGF165 induces strong endothelial cell proliferation, whereas bFGF elicits fibrocyte proliferation and minor endothelial cell proliferation. Heparin induces squamous metaplasia of the chorionic and allantoic epithelium in combination with an aggregation of fibrocytes. We could not detect any enhancement of VEGF165 by heparin.
研究了血管内皮生长因子（VEGF165）对13日龄鸡胚绒毛尿囊膜（CAM）的影响。该因子以0.5 - 4微克的剂量应用长达4天。进行了宏观、组织学和免疫组织学研究。用抗VEGF抗体检测该因子的定位。通过BrdU -抗BrdU法研究VEGF165和碱性成纤维细胞生长因子（bFGF）的促有丝分裂活性。此外，还研究了单独使用肝素以及肝素与VEGF165联合使用的效果。VEGF165在0.5微克及以上剂量时特异性诱导血管生成。在毛细血管前血管区域可见血管呈刷状形成。在毛细血管和小静脉区域也发生血管生成。组织学上，我们发现了出芽以及套入性毛细血管生长的迹象。用抗VEGF抗体可在3天内证明应用区域存在该因子。该因子位于绒毛膜上皮和上皮内毛细血管中。BrdU研究表明，VEGF165诱导强烈的内皮细胞增殖，而bFGF引发成纤维细胞增殖和轻微的内皮细胞增殖。肝素与成纤维细胞聚集一起诱导绒毛膜和尿囊上皮的鳞状化生。我们未检测到肝素对VEGF165有任何增强作用。
