Nakai T, Kawahara K, Horiguchi Y, Danbara H, Kume K
Research Center for Veterinary Science, Kitasato Institute, Tokyo, Japan.
Am J Vet Res. 1992 Sep;53(9):1519-23.
Two monoclonal antibodies against Actinobacillus pleuropneumoniae serotype 5, designated as 5MAb-1 and 5MAb-6, were characterized. Enzyme-linked immunosorbent assay-inhibition tests with whole-cell antigens obtained from strains of serotype 1 through 12 of A pleuropneumoniae revealed that 5 MAb-1 bound to only serotype-5 strains. The epitope recognized by 5MAb-1 was a carbohydrate that was sensitive to periodate oxidation and resided on the structure of beta-1,6-linked D-galactose in an O-antigen polysaccharide of serotype-5 lipopolysaccharide. Analysis of these results revealed that the O-antigen polysaccharide of lipopolysaccharide was 1 of the antigenic determinants responsible for the serotype specificity of A pleuropneumoniae. On the other hand, 5MAb-6 reacted with strains of serotype 1 through 10 in varying degrees and its epitope was located on polypeptides sensitive to proteinase K. In an immunoblotting analysis, 5MAb-6 reacted with 2 polypeptide bands, with molecular weights of approximately 41,500 and 28,000, in the outer membrane protein-rich fraction obtained from strains of serotype 1 through 10. These results indicated that outer membrane proteins from several serotype strains of A pleuropneumoniae possessed common antigenic determinants.
对两种针对胸膜肺炎放线杆菌血清型5的单克隆抗体（分别命名为5MAb-1和5MAb-6）进行了特性分析。用从胸膜肺炎放线杆菌血清型1至12菌株获得的全细胞抗原进行酶联免疫吸附抑制试验，结果显示5MAb-1仅与血清型5菌株结合。5MAb-1识别的表位是一种对高碘酸盐氧化敏感的碳水化合物，位于血清型5脂多糖O抗原多糖中β-1,6-连接的D-半乳糖结构上。对这些结果的分析表明，脂多糖的O抗原多糖是胸膜肺炎放线杆菌血清型特异性的抗原决定簇之一。另一方面，5MAb-6与血清型1至10的菌株有不同程度的反应，其表位位于对蛋白酶K敏感的多肽上。在免疫印迹分析中，5MAb-6与从血清型1至10菌株获得的富含外膜蛋白的组分中的两条多肽带发生反应，其分子量约为41,500和28,000。这些结果表明，胸膜肺炎放线杆菌几个血清型菌株的外膜蛋白具有共同的抗原决定簇。
