胸膜肺炎放线杆菌5b型单克隆抗体的制备与鉴定
Production and characterization of monoclonal antibodies to Actinobacillus pleuropneumoniae serotype 5b.
作者信息
Altman E, Gidney M A, Harrison B A, Gottschalk M
机构信息
Institute for Biological Sciences, National Research Council of Canada Ottawa, Ontario, Canada.
出版信息
Can J Vet Res. 1995 Oct;59(4):279-84.
Monoclonal antibodies specific for capsular polysaccharide (CPS) and lipopolysaccharide (LPS) of A. pleuropneumoniae serotype 5b were generated by hybridoma cells and selected by indirect ELISA of culture supernatants with purified and structurally defined LPS and CPS preparations and their synthetic conjugates. It was shown in this study that at least one monoclonal antibody, 3B4, presented 100% specificity and recognized all A. pleuropneumoniae serotype 5 field strains tested in a dot-ELISA assay.
通过杂交瘤细胞产生了针对胸膜肺炎放线杆菌5b型荚膜多糖（CPS）和脂多糖（LPS）的单克隆抗体，并通过用纯化的、结构明确的LPS和CPS制剂及其合成缀合物对培养上清液进行间接ELISA筛选。本研究表明，至少一种单克隆抗体3B4具有100%的特异性，并且在斑点ELISA试验中能识别所有测试的胸膜肺炎放线杆菌5型野毒株。
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