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胸膜肺炎放线杆菌5b型单克隆抗体的制备与鉴定

Production and characterization of monoclonal antibodies to Actinobacillus pleuropneumoniae serotype 5b.

作者信息

Altman E, Gidney M A, Harrison B A, Gottschalk M

机构信息

Institute for Biological Sciences, National Research Council of Canada Ottawa, Ontario, Canada.

出版信息

Can J Vet Res. 1995 Oct;59(4):279-84.

PMID:8548689
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1263782/
Abstract

Monoclonal antibodies specific for capsular polysaccharide (CPS) and lipopolysaccharide (LPS) of A. pleuropneumoniae serotype 5b were generated by hybridoma cells and selected by indirect ELISA of culture supernatants with purified and structurally defined LPS and CPS preparations and their synthetic conjugates. It was shown in this study that at least one monoclonal antibody, 3B4, presented 100% specificity and recognized all A. pleuropneumoniae serotype 5 field strains tested in a dot-ELISA assay.

摘要

通过杂交瘤细胞产生了针对胸膜肺炎放线杆菌5b型荚膜多糖（CPS）和脂多糖（LPS）的单克隆抗体，并通过用纯化的、结构明确的LPS和CPS制剂及其合成缀合物对培养上清液进行间接ELISA筛选。本研究表明，至少一种单克隆抗体3B4具有100%的特异性，并且在斑点ELISA试验中能识别所有测试的胸膜肺炎放线杆菌5型野毒株。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b4e/1263782/18bf21655d06/cjvetres00028-0044-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b4e/1263782/95d7bffafc1c/cjvetres00028-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b4e/1263782/18bf21655d06/cjvetres00028-0044-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b4e/1263782/95d7bffafc1c/cjvetres00028-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b4e/1263782/18bf21655d06/cjvetres00028-0044-b.jpg

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本文引用的文献

1
Detection of Actinobacillus pleuropneumoniae in the porcine upper respiratory tract as a complement to serological tests.检测猪上呼吸道中的胸膜肺炎放线杆菌作为血清学检测的补充
Can J Vet Res. 1993 Jul;57(3):204-8.
2
Identification of Actinobacillus pleuropneumoniae serotype 2 by monoclonal or polyclonal antibodies in latex agglutination tests.在乳胶凝集试验中通过单克隆或多克隆抗体鉴定胸膜肺炎放线杆菌2型。
Acta Vet Scand. 1993;34(2):223-5. doi: 10.1186/BF03548215.
3
Evaluation of long chain lipopolysaccharides (LC-LPS) of Actinobacillus pleuropneumoniae serotype 5 for the serodiagnosis of swine pleuropneumonia.
胸膜肺炎放线杆菌5型长链脂多糖用于猪胸膜肺炎血清学诊断的评估
Vet Microbiol. 1994 Feb;38(4):315-27. doi: 10.1016/0378-1135(94)90150-3.
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Serotyping and detection of Haemophilus pleuropneumoniae by indirect fluorescent antibody technique.应用间接荧光抗体技术对猪胸膜肺炎放线杆菌进行血清型鉴定及检测
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Immunochemistry of groups A, B, and C meningococcal polysaccharide-tetanus toxoid conjugates.A、B和C群脑膜炎球菌多糖-破伤风类毒素结合物的免疫化学
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A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中脂多糖的灵敏银染法。
Anal Biochem. 1982 Jan 1;119(1):115-9. doi: 10.1016/0003-2697(82)90673-x.
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Maturation of the head of bacteriophage T4. I. DNA packaging events.噬菌体T4头部的成熟。I. DNA包装事件。
J Mol Biol. 1973 Nov 15;80(4):575-99. doi: 10.1016/0022-2836(73)90198-8.
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High-molecular-weight components in lipopolysaccharides of Salmonella typhimurium, Salmonella minnesota, and Escherichia coli.鼠伤寒沙门氏菌、明尼苏达沙门氏菌和大肠杆菌脂多糖中的高分子量成分。
J Bacteriol. 1985 May;162(2):738-45. doi: 10.1128/jb.162.2.738-745.1985.
10
An evaluation of agglutination and coagglutination techniques for serotyping of Haemophilus pleuropneumoniae isolates.对胸膜肺炎放线杆菌分离株进行血清分型的凝集和协同凝集技术评估。
Am J Vet Res. 1987 Feb;48(2):219-26.