通过逆转录聚合酶链反应（RT-PCR）检测分离的大鼠肾小管中的内皮素-1信使核糖核酸（mRNA）。

Detection of endothelin-1 mRNA by RT-PCR in isolated rat renal tubules.

作者信息

Uchida S, Takemoto F, Ogata E, Kurokawa K

机构信息

Fourth Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Biochem Biophys Res Commun. 1992 Oct 15;188(1):108-13. doi: 10.1016/0006-291x(92)92356-3.

DOI:10.1016/0006-291x(92)92356-3

PMID:1384475

Abstract

A combination of reverse transcription and polymerase chain reaction was utilized to detect baseline endothelin-1 (ET-1) mRNA expressed in renal tubules dissected from rats. 5' and 3' primers were constructed according to human ET-1 genomic DNA. Rat ET-1 mRNA was found to be expressed only in cortical through medullary collecting ducts in addition to glomeruli. Sites for tubular synthesis of ET-1 corroborate well with major ET-1 binding sites along the nephron, indicating autocrine/paracrine role of ET-1 in the renal tubules and supporting a prevailing concept on such function of ET-1 in many differing tissues.

摘要

采用逆转录和聚合酶链反应相结合的方法，检测从大鼠分离的肾小管中表达的基线内皮素-1（ET-1）mRNA。根据人ET-1基因组DNA构建5'和3'引物。发现大鼠ET-1 mRNA除了在肾小球中表达外，还仅在皮质至髓质集合管中表达。ET-1的肾小管合成部位与沿肾单位的主要ET-1结合位点高度吻合，表明ET-1在肾小管中具有自分泌/旁分泌作用，并支持ET-1在许多不同组织中具有这种功能的普遍概念。