用阳离子化免疫球蛋白G免疫的兔子体内产生的抗体可与组蛋白H3和H4发生反应。
Antibodies in rabbits immunized with cationized IgG react with histones H3 and H4.
作者信息
Suzuki T, Burlingame R W, Cavalot F, Andres G, Kashiwazaki S, Tan E M
机构信息
W. M. Keck Autoimmune Disease Center, La Jolla, California.
出版信息
Arthritis Rheum. 1992 Oct;35(10):1218-26. doi: 10.1002/art.1780351015.
OBJECTIVE
Rabbits immunized with cationized rabbit IgG develop antinuclear antibodies. This study was aimed at identifying the reactive antigens.
METHODS
Rabbits were immunized with homotypic or allotypic IgG that were physicochemically altered to produce positively charged dimethylpropanamide (polycationic) side chains.
RESULTS
Seven of 11 rabbits injected with cationized IgG produced antinuclear antibodies detected by indirect immunofluorescence. By enzyme-linked immunosorbent assay, these were IgG antibodies reacting with histone (H3-H4)2 tetramer and with individual histone polypeptides H3 and H4. The antihistone antibodies were absorbed by cationized IgG but not by normal IgG.
CONCLUSION
Cationized IgG appears to possess antigenic determinants of sufficient similarity to produce antibody responses cross-reactive with histones H3 and H4.
目的
用阳离子化兔IgG免疫的兔子会产生抗核抗体。本研究旨在鉴定反应性抗原。
方法
用经物理化学改变以产生带正电荷的二甲基丙酰胺（聚阳离子）侧链的同型或异型IgG免疫兔子。
结果
11只注射阳离子化IgG的兔子中有7只产生了通过间接免疫荧光检测到的抗核抗体。通过酶联免疫吸附测定，这些是与组蛋白（H3-H4）2四聚体以及单个组蛋白多肽H3和H4反应的IgG抗体。抗组蛋白抗体被阳离子化IgG吸收，但不被正常IgG吸收。
结论
阳离子化IgG似乎具有足够相似的抗原决定簇，以产生与组蛋白H3和H4交叉反应的抗体反应。
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