Monestier M, Losman J A, Fasy T M, Debbas M E, Massa M, Albani S, Bohm L, Martini A
Center for Molecular Medicine and Immunology, Newark, New Jersey 07103.
Arthritis Rheum. 1990 Dec;33(12):1836-41. doi: 10.1002/art.1780331212.
The binding of antinuclear antibody-positive juvenile arthritis (JA) sera to bovine thymus histones H1, H2A, H2B, H3, and H4 was studied by an enzyme-linked immunosorbent assay. Seventy-five percent of the JA patients tested positive for at least 1 antibody specificity. Antihistone antibodies were predominantly IgM, while IgG antibodies were less common and were restricted to histones H1 or H3. In the group of patients with JA of pauciarticular onset, antihistone antibodies were significantly more elevated in patients with past or present uveitis than in patients without a history of uveitis. Anti-H1 antibodies in JA patients were found to react mostly with determinants located in the carboxyl-terminal domain of the H1 molecule. Sera were also reactive with human histone H1(0) or chicken histone H5, which are H1 variants found only in nondividing cells.
采用酶联免疫吸附测定法研究了抗核抗体阳性的青少年关节炎（JA）血清与牛胸腺组蛋白H1、H2A、H2B、H3和H4的结合情况。75%的受试JA患者至少对1种抗体特异性呈阳性反应。抗组蛋白抗体主要为IgM，而IgG抗体较少见，且仅限于组蛋白H1或H3。在少关节起病的JA患者组中，有既往或现患葡萄膜炎的患者抗组蛋白抗体水平显著高于无葡萄膜炎病史的患者。发现JA患者的抗H1抗体大多与位于H1分子羧基末端结构域的决定簇发生反应。血清也与仅在非分裂细胞中发现的H1变体人组蛋白H1(0)或鸡组蛋白H5发生反应。
