Choppin J, Guillet J G, Lévy J P
Institut Cochin de Génétique Moléculaire (ICGM), INSERM U152, Paris, France.
Crit Rev Immunol. 1992;12(1-2):1-16.
To identify HIV peptides containing HLA class I binding regions, different studies have been performed. These include the detection of interactions between HIV peptides and purified HLA molecules in solid-phase assays, the measurement of HLA molecule assembly in the presence of peptide added to cell lysates, and the detection of inhibition of CTL-mediated cytolysis by competition between peptides on target cells. To date, the HIV epitopes recognized by anti-HIV CTL are from the Env, Gag, Nef, and Pol proteins and they are identified using synthetic peptides of 12 to 20 amino acids. The search for a correlation between known HIV CTL epitopes and the results of HLA/peptide interaction assays reveals that: (1) most of the peptides that are positive in the assembly assay contain a HLA-A2 peptide motif but the correlation between these positive peptides and the CTL epitopes is not obvious; (2) a high proportion of HIV epitopes are included in the peptides positive in solid-phase binding and in inhibition of cytolysis assays, although these tests do not allow us to predict HLA restriction; (3) the HLA-A2 peptide motif is not systematically included in HLA-A2-restricted CTL epitopes, this observation raising the possibility that other sequences are involved in HLA binding.
为了鉴定含有HLA I类结合区的HIV肽段，人们开展了不同的研究。这些研究包括在固相分析中检测HIV肽段与纯化的HLA分子之间的相互作用、在向细胞裂解物中添加肽段的情况下测量HLA分子组装，以及通过靶细胞上肽段之间的竞争来检测CTL介导的细胞溶解的抑制作用。迄今为止，抗HIV CTL识别的HIV表位来自Env、Gag、Nef和Pol蛋白，并且它们是使用12至20个氨基酸的合成肽段来鉴定的。对已知HIV CTL表位与HLA/肽段相互作用分析结果之间的相关性进行研究后发现：（1）在组装分析中呈阳性的大多数肽段含有HLA-A2肽基序，但这些阳性肽段与CTL表位之间的相关性并不明显；（2）尽管这些检测无法让我们预测HLA限制，但固相结合和细胞溶解抑制分析中呈阳性的肽段中包含高比例的HIV表位；（3）HLA-A2肽基序并未系统性地包含在HLA-A2限制的CTL表位中，这一观察结果增加了其他序列参与HLA结合的可能性。
