Choppin J, Martinon F, Connan F, Pauchard M, Gomard E, Levy J P
Institut Cochin de Génétique Moléculaire (ICGM), INSERM U152, Paris, France.
J Immunol. 1991 Jul 15;147(2):575-83.
To detect HLA-binding peptides in 10 HIV-1 proteins (Rev, Tat, Vif, Vpr, Vpu, Gag p18, Gag p24, Gag p15, Env gp120 and Env gp41), the peptide binding assay (PBA) has been performed using three HLA class I molecules. Correlations have been searched between the PBA results and the peptide competitor activity in a functional test using HLA-A2-restricted CTL and target cells. A correlation between the data found in the PBA and well-defined CTL epitopes could be attempted only for the three Gag proteins. For these proteins, our results are in agreement with the known existence of epitopes reacting with human CD8+ CTL, with some exceptions. Together with the results reported with a panel of Nef peptides, these experiments showed that at least 18/20 of the already reported CTL epitopes from HIV-1 Gag, Nef, and Env proteins could be detected by the PBA, most (17/18) corresponding to strong reactivities. Perhaps more important, the regions of HIV-1 Gag p24 or Nef proteins that contain multiple associated CTL epitopes, with different HLA restrictions, were clearly identified by the reactivities in the PBA of several overlapping peptides and the major practical interest of the PBA might be the detection of such polyepitopic regions. Prediction are proposed in this report for 10 proteins, including several proteins for which CTL epitopes remain presently unknown.
为了检测10种HIV-1蛋白（Rev、Tat、Vif、Vpr、Vpu、Gag p18、Gag p24、Gag p15、Env gp120和Env gp41）中的HLA结合肽，使用三种HLA I类分子进行了肽结合试验（PBA）。在使用HLA-A2限制性CTL和靶细胞的功能试验中，研究了PBA结果与肽竞争活性之间的相关性。仅对三种Gag蛋白尝试了PBA中发现的数据与明确的CTL表位之间的相关性。对于这些蛋白，我们的结果与已知存在的与人CD8 + CTL反应的表位一致，但有一些例外。连同一组Nef肽的报告结果，这些实验表明，PBA可以检测出至少18/20已报道的来自HIV-1 Gag、Nef和Env蛋白的CTL表位，大多数（17/18）对应于强反应性。也许更重要的是，通过几种重叠肽在PBA中的反应性，清楚地确定了HIV-1 Gag p24或Nef蛋白中包含多个具有不同HLA限制性相关CTL表位的区域，PBA的主要实际意义可能在于检测此类多表位区域。本报告对10种蛋白提出了预测，包括几种目前CTL表位尚不清楚的蛋白。
