Rouet P, Daveau M, Salier J P
Institut National de la Santé et de la Recherche Médicale Unit-78, Boisguillaume, France.
Biol Chem Hoppe Seyler. 1992 Oct;373(10):1019-24. doi: 10.1515/bchm3.1992.373.2.1019.
The inter-alpha-inhibitor (I alpha I) family encompasses four plasma proteins, namely free bikunin as well as I alpha I, pre-alpha-inhibitor (P alpha I) and inter-alpha-like inhibitor (I alpha LI). Each of the last three proteins is a distinct assembly of one bikunin chain with one or two out of three distinct, heavy (H) chains designated H1, H2 and H3. I alpha I is made of [H1 + H2 + bikunin], P alpha I is made of [H3 + bikunin] and I alpha LI is made of [H2 + bikunin]. We characterized various I alpha I family-related antisera/antibodies, including a reagent recognizing bikunin, another directed against the four H1, H2, H3 and bikunin chains, and a third one directed against both H1 and H2 chains. By a simplified absorption procedure of the latter two antisera onto solid-phase immunoadsorbents made with i) purified I alpha I, or ii) a recombinant H1 polypeptide, we also obtained specific anti-H3 or anti-H2 antibodies, respectively. This series of new antibodies was used to identify each component within the pattern of bands corresponding to I alpha I family in human serum, as separated by SDS-PAGE and western blotting.
α-间抑制因子（IαI）家族包含四种血浆蛋白，即游离比基尼以及IαI、前α-抑制因子（PαI）和α-间样抑制因子（IαLI）。后三种蛋白中的每一种都是由一条比基尼链与三种不同的重链（H）中的一条或两条组成的独特组合，这三种重链分别命名为H1、H2和H3。IαI由[H1 + H2 + 比基尼]组成，PαI由[H3 + 比基尼]组成，IαLI由[H2 + 比基尼]组成。我们对各种与IαI家族相关的抗血清/抗体进行了表征，包括一种识别比基尼的试剂、另一种针对H1、H2、H3和比基尼四条链的试剂，以及第三种针对H1和H2两条链的试剂。通过将后两种抗血清用i）纯化的IαI或ii）重组H1多肽制成的固相免疫吸附剂进行简化吸收程序，我们还分别获得了特异性抗H3或抗H2抗体。这一系列新抗体用于识别在人血清中经SDS-PAGE和western印迹分离后与IαI家族相对应的条带模式中的每个成分。
