血清间α-胰蛋白酶抑制剂（ITI）家族各成员中三种间α-胰蛋白酶抑制剂重链的参与情况。

Involvement of the three inter-alpha-trypsin inhibitor (ITI) heavy chains in each member of the serum ITI family.

作者信息

Héron A, Bourguignon J, Diarra-Mehrpour M, Dautréaux B, Martin J P, Sesboüé R

机构信息

INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, St. Etienne-Rouvray, France.

出版信息

FEBS Lett. 1995 Oct 30;374(2):195-8. doi: 10.1016/0014-5793(95)01103-l.

DOI:10.1016/0014-5793(95)01103-l

PMID:7589533

Abstract

Partial cDNAs coding for each of the three human inter-alpha-trypsin inhibitor (ITI) heavy chains were expressed in a bacterial plasmid system and rabbits were immunised with the fusion peptides obtained. Despite the strong sequence homology of these chains, the antisera turned out to be highly specific in the analysis of corresponding mRNA translation products or partially digested serum ITI. Besides classical serum ITI members, their use in Western blotting made it possible to evidence an H3-related ITI form and a low-amount H1-related HC/bikunin component. The relative levels of ITI family members was further studied in baboon and foetal calf sera.

摘要

编码三种人α-胰蛋白酶抑制剂（ITI）重链的部分cDNA在细菌质粒系统中表达，并将获得的融合肽免疫兔子。尽管这些链具有很强的序列同源性，但抗血清在分析相应的mRNA翻译产物或部分消化的血清ITI时具有高度特异性。除了经典的血清ITI成员外，它们在蛋白质免疫印迹中的应用使得鉴定一种与H3相关的ITI形式和一种低含量的与H1相关的重链/抑肽酶成分成为可能。在狒狒和胎牛血清中进一步研究了ITI家族成员的相对水平。