Szabó I, Petronilli V, Zoratti M
CNR Unit for the Physiology of Mitochondria, Department of Biomedical Sciences, Padova, Italy.
Biochim Biophys Acta. 1992 Nov 23;1112(1):29-38. doi: 10.1016/0005-2736(92)90250-p.
In patch-clamp experiments on giant protoplasts of the Gram-positive bacterium Bacillus subtilis, membrane stretch resulted in an initial transient collapse of the membrane resistance, after which stretch-activated, voltage modulated, high-conductance channels could be observed. The channel open probability increased exponentially with applied suction and positive voltage, as a result of variations of both the mean open and the mean closed times. The substate structure and other characteristics of the electrical activity suggested the presence of a family of pores exhibiting cooperative behavior. A role in osmotic protection is suggested. In the intact bacteria, the pores may be part of an unidentified envelope apparatus, having other functions as well.
在对革兰氏阳性细菌枯草芽孢杆菌的巨大原生质体进行膜片钳实验时，膜拉伸导致膜电阻最初出现短暂崩溃，之后可观察到拉伸激活、电压调制的高电导通道。由于平均开放时间和平均关闭时间的变化，通道开放概率随施加的吸力和正电压呈指数增加。电活动的亚状态结构和其他特征表明存在一类表现出协同行为的孔道。有人提出其具有渗透保护作用。在完整细菌中，这些孔道可能是一种尚未明确的包膜装置的一部分，还具有其他功能。
