Szabó I, Petronilli V, Zoratti M
CNR Unit, Department of Biomedical Sciences, Padova, Italy.
J Membr Biol. 1993 Feb;131(3):203-18. doi: 10.1007/BF02260109.
The patch-clamp technique was used to study the membrane of giant protoplasts from the gram-positive bacterium Streptococcus faecalis, demonstrating the presence of ion-conducting pores in the cytoplasmic membrane of procaryotes. The single channel recordings were characterized by a variety of conductances, ranging up to a few nanoSiemens, arising from stretch-activated, voltage-modulated, cooperative channels. Activation by stretch and voltage took place via both a decrease of the mean closed time and an increase of the mean open time of the channels, which are strictly controlled in intact cells, where they might constitute parts of a membrane apparatus or transport system.
采用膜片钳技术研究革兰氏阳性粪肠球菌巨大原生质体的膜，证明原核生物细胞质膜中存在离子传导孔。单通道记录的特征是具有多种电导，高达几纳西门子，这些电导来自拉伸激活、电压调制的协同通道。拉伸和电压激活是通过通道平均关闭时间的减少和平均开放时间的增加来实现的，这些在完整细胞中受到严格控制，在完整细胞中它们可能构成膜装置或运输系统的一部分。
