McDonald A J
Department of Cell Biology and Neuroscience, University of South Carolina School of Medicine, Columbia 29208.
Neuroreport. 1992 Oct;3(10):821-7. doi: 10.1097/00001756-199210000-00001.
Recent studies have shown that biocytin may have multiple applications in neuroanatomical studies. Biocytin may be injected into the brain by iontophoresis or by pressure injection methods, and localized in tissue sections using avidin-conjugated labels. It is taken up by neurons and rapidly transported down axons in an anterograde fashion. Axons are completely labeled in a Golgi-like manner and can be examined at both light and electron microscopic levels. Biocytin can also be used in retrograde tract tracing experiments, although in some cases it appears that fibers must be damaged to produce such labeling. Retrogradely labeled cells may be completely labeled, resembling neurons stained with the Golgi technique. Individual neurons can also be labeled in a Golgi-like manner by uptake of biocytin from the extracellular space. Thus, it appears that biocytin is an especially versatile marker for neuroanatomical investigations.
最近的研究表明，生物胞素在神经解剖学研究中可能有多种应用。生物胞素可通过离子电泳或压力注射法注入大脑，并使用抗生物素蛋白偶联标记物在组织切片中定位。它被神经元摄取，并以顺行方式迅速沿轴突运输。轴突以类似高尔基染色法的方式被完全标记，并且可以在光学显微镜和电子显微镜水平上进行检查。生物胞素也可用于逆行束路追踪实验，尽管在某些情况下，似乎必须损伤纤维才能产生这种标记。逆行标记的细胞可能被完全标记，类似于用高尔基技术染色的神经元。单个神经元也可以通过从细胞外空间摄取生物胞素，以类似高尔基染色法的方式进行标记。因此，生物胞素似乎是神经解剖学研究中一种特别通用的标记物。
