King M A, Louis P M, Hunter B E, Walker D W
Gainesville Veterans Administration Medical Center, FL.
Brain Res. 1989 Sep 18;497(2):361-7. doi: 10.1016/0006-8993(89)90281-3.
Biocytin, a naturally occurring low molecular weight analog of biotin, was evaluated as a neuroanatomical tract-tracing marker in the adult rat brain. Since it retains high-affinity binding to avidin, biocytin can be labelled with avidinylated visualization reagents. Iontophoretic or pressure injections resulted in filling of cell bodies and dendrites around the injection site and their efferent axonal processes and boutons. Retrogradely labelled neurons were occasionally observed at a distance but only with large injections. Anterograde tracing with biocytin is successful even in animals that are quite old, in contrast to lectins and HRP conjugates, and offers advantages in delivery, tissue processing, selection of light and/or electron microscopic labels, time to obtain results, and cost over many conventional tracers.
生物胞素是一种天然存在的生物素低分子量类似物，被评估为成年大鼠脑中的神经解剖学示踪标记物。由于它与抗生物素蛋白保持高亲和力结合，生物胞素可用抗生物素蛋白化的可视化试剂进行标记。离子电渗法或压力注射导致注射部位周围的细胞体和树突及其传出轴突和终扣被填充。偶尔在一定距离处观察到逆行标记的神经元，但仅在大剂量注射时出现。与凝集素和辣根过氧化物酶偶联物相比，用生物胞素进行顺行示踪即使在非常年老的动物中也很成功，并且在递送、组织处理、光镜和/或电镜标记的选择、获得结果的时间以及成本方面比许多传统示踪剂具有优势。
