Elfvin L G, Aldskogius H, Johansson J
Department of Anatomy, Karolinska Institutet, Stockholm, Sweden.
Cell Tissue Res. 1992 Aug;269(2):229-34. doi: 10.1007/BF00319613.
The distribution of primary visceral afferents to the spleen of the guinea pig was studied after injections of wheat-germ agglutinin conjugated to horseradish peroxidase (HRP) into the left dorsal root ganglia at levels T7-T12. After anterograde transport of the tracer, labeled fibers were found in the nerves around the splenic artery in the hilus region and in the splenic parenchyma. The majority of labeled fibers in the spleen were detected in the white pulp. HRP-positive fibers were also observed in the red pulp and in the trabeculae. The distribution of the HRP-labeled fibers was in part similar to those of substance P-immunoreactive and calcitonin gene-related peptide-immunoreactive nerve structures. The results show that the anterograde tracing technique can be used successfully to investigate splenic primary afferent innervation.
在豚鼠T7 - T12水平的左侧背根神经节注射与辣根过氧化物酶（HRP）结合的麦胚凝集素后，研究了初级内脏传入神经向豚鼠脾脏的分布。在示踪剂进行顺行运输后，在脾门区脾动脉周围的神经以及脾实质中发现了标记纤维。脾脏中大多数标记纤维见于白髓。在红髓和小梁中也观察到了HRP阳性纤维。HRP标记纤维的分布部分类似于P物质免疫反应性和降钙素基因相关肽免疫反应性神经结构的分布。结果表明，顺行追踪技术可成功用于研究脾脏初级传入神经支配。
