Li X G, Somogyi P, Tepper J M, Buzsáki G
Center for Molecular and Behavioral Neuroscience, Rutgers, State University of New Jersey, Newark 07102.
Exp Brain Res. 1992;90(3):519-25. doi: 10.1007/BF00230934.
During the course of an in vivo intracellular labeling study, a chandelier (axo-axonic) cell was completely filled with biocytin in the CA1 region of the hippocampus. Chandelier cells are known to provide GABAergic terminals exclusively to the axon initial segment of pyramidal cells. The lateral extent and laminar distribution of the dendritic arborization of the chandelier cell was very similar to that of pyramidal cells; the numerous basal and apical dendrites reached the ventricular surface and the hippocampal fissure, respectively. The dendrites, however, had very few spines. The neuron had an asymmetric axonal arbor occupying an elliptical area of 600 by 850 microns in the pyramidal cell layer and stratum oriens, with over three-quarters of the axon projecting to the fimbrial side of the neuron. Counting all clusters of terminals, representing individually innervated axon initial segments, the chandelier cell was estimated to contact 1214 pyramidal cells, a number that exceeds previous estimations, based on Golgi studies, by several-fold. The findings support the view that chandelier cells may control the threshold and/or synchronize large populations of principal cells.
在一项体内细胞内标记研究过程中，在海马体CA1区，一个吊灯（轴突 - 轴突型）细胞被生物素完全填充。已知吊灯细胞仅向锥体细胞的轴突起始段提供GABA能终末。吊灯细胞树突分支的横向范围和层状分布与锥体细胞非常相似；众多的基底树突和顶树突分别到达脑室表面和海马裂。然而，这些树突上的棘很少。该神经元有一个不对称的轴突分支，在锥体细胞层和 Oriens 层占据一个600×850微米的椭圆形区域，超过四分之三的轴突投射到神经元的海马伞侧。计算所有代表单个受支配轴突起始段的终末簇，估计该吊灯细胞与1214个锥体细胞接触，这一数字比基于高尔基染色研究的先前估计值高出几倍。这些发现支持了这样一种观点，即吊灯细胞可能控制大量主细胞的阈值和/或使其同步。
