Shuler C F, Halpern D E, Guo Y, Sank A C
Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles 90033.
Dev Biol. 1992 Dec;154(2):318-30. doi: 10.1016/0012-1606(92)90071-n.
Vital cell labeling techniques were used to trace the fate of the medial edge epithelial (MEE) cells during palatal fusion in vivo. Mouse palatal tissues were labeled in utero with DiI. The fetuses continued to develop in utero and tissues of the secondary palate were examined at several later stages of palatal ontogeny. The presence and distribution of DiI was correlated with the presence of cell phenotype-specific markers. During the initial stages of palatal fusion the DiI-labeled MEE were present in the midline position. These cells were attached to an intact laminin-containing basement membrane and contained keratin intermediate filaments. At later stages of palatogenesis the DiI-labeled MEE were not separated from the mesenchyme by an intact basement membrane and did not contain keratin. In late fetal development, DiI-labeled cells without an epithelial morphology were present in the mesenchyme. The transition of the DiI-labeled cells from an epithelial phenotype to a mesenchymal phenotype is consistent with a fate of epithelial-mesenchymal transformation rather than programmed cell death.
运用重要细胞标记技术在体内追踪腭融合过程中内侧边缘上皮（MEE）细胞的命运。在子宫内用DiI标记小鼠腭组织。胎儿在子宫内继续发育，并在腭发育的几个后期阶段检查继发腭的组织。DiI的存在和分布与细胞表型特异性标记物的存在相关。在腭融合的初始阶段，DiI标记的MEE位于中线位置。这些细胞附着于完整的含层粘连蛋白的基底膜上，并含有角蛋白中间丝。在腭发生的后期阶段，DiI标记的MEE未被完整的基底膜与间充质分开，且不含角蛋白。在胎儿发育后期，间充质中存在无上皮形态的DiI标记细胞。DiI标记细胞从上皮表型向间充质表型的转变与上皮-间充质转化的命运一致，而非程序性细胞死亡。
