Olivereau M, Olivereau J M
Laboratorie de Physiologie, Institut Océanographique, Paris, France.
Histochemistry. 1992 Aug;98(1):51-66. doi: 10.1007/BF00716937.
Galanin-like immunoreactivity was localized in the brain of Urodela (Ambystoma, Pleurodeles) and Anura (Bufo, Xenopus) by immunocytochemistry with anti-porcine galanin antiserum. In the four species, immunoreactive perikarya were observed in the telencephalon (striatum, amygdala), diencephalon preoptic area mainly along the anterodorsal wall of the preoptic recessus, suprachiasmatic nucleus, lateral hypothalamus, ventral and dorsal infundibular nuclei, paraventricular organ, and rhombencephalon (nucleus of the solitary tract). Galaninergic fibres extended in similar regions and in the medial septum, ventral telencephalon, ventral hypothalamus, median eminence, and various mesencephalic and rhombencephalic regions. Contacts with the cerebrospinal fluid cavity occurred along the preoptic recessus (Ambystoma) and the ventral infundibular wall (all species). Fibres were scarce in the neurohypophysis. The distal and intermediate lobes of the pituitary were virtually devoid of immunoreactivity. The galaninergic system appeared more developed in adult amphibia than in young animals, suggesting the stimulating influence of sex steroids on the expression of galanin as previously described in Anguilla. The extensive distribution of the galanin-like immunoreactive neurons in amphibian brains suggests that this peptide may act as a neuromodulatur and/or neurotransmitter.
运用抗猪甘丙肽抗血清进行免疫细胞化学研究，发现甘丙肽样免疫反应性定位于有尾目（蝾螈、肋突螈）和无尾目（蟾蜍、非洲爪蟾）动物的大脑中。在这四个物种中，免疫反应性核周体见于端脑（纹状体、杏仁核）、间脑视前区，主要沿视前隐窝的前背壁、视交叉上核、下丘脑外侧区、腹侧和背侧漏斗核、室旁器官以及后脑（孤束核）。甘丙肽能纤维在类似区域以及内侧隔、腹侧端脑、腹侧下丘脑、正中隆起以及各种中脑和后脑区域延伸。在视前隐窝（蝾螈）和腹侧漏斗壁（所有物种）处有与脑脊液腔的接触。神经垂体中的纤维稀少。垂体的远侧叶和中间叶几乎没有免疫反应性。与幼体动物相比，甘丙肽能系统在成年两栖动物中似乎更为发达，这表明性类固醇对甘丙肽表达具有刺激作用，正如先前在鳗鲡中所描述的那样。甘丙肽样免疫反应性神经元在两栖动物大脑中的广泛分布表明，这种肽可能作为一种神经调质和/或神经递质发挥作用。
