Scibilia R J, Lachowicz J E, Kilts C D
Department of Psychiatry, Duke University Medical Center, Durham, North Carolina 27710.
Synapse. 1992 Jun;11(2):146-54. doi: 10.1002/syn.890110208.
The distribution of D1 and D2 dopamine (DA) receptors in the nuclei and subnuclear zones of the rat amygdaloid complex was mapped using quantitative light microscopic autoradiography. [125I]iodosulpiride and [125I]SCH 23982 (in the presence of 50 nM ketanserin) were used to label D2 and D1 DA receptors, respectively. The DA receptor subtypes exhibited a topographic, nonoverlapping distribution which generally conformed to the cytoarchitectonic boundaries of the component nuclei and subnuclear zones of the amygdaloid complex. The highest density of [125I]iodosulpiride binding sites was observed in the main intercalated cell group and the central amygdaloid nucleus where a medial to lateral gradient of binding sites was localized to its subnuclear zones. [125I]SCH 23982 binding sites were localized in the main intercalated cell group and the basolateral amygdaloid nucleus with a uniform low density in the central nucleus. The functional topography of mesoamygdaloid DA neurons may therefore be mediated, in part, at the level of DA receptor subtypes. The pattern of distribution of [125I]iodosulpiride binding sites in subdivisions of the central amygdaloid nucleus and bed nucleus of the stria terminalis suggests that the functions of the "extended amygdala," a major system of the functional organization of the basal forebrain, may be regulated by DA afferents at multiple key sites of D2 receptor action.
利用定量光学显微镜放射自显影技术绘制了大鼠杏仁核复合体各核团及亚核区中D1和D2多巴胺（DA）受体的分布图。分别使用[125I]碘舒必利和[125I]SCH 23982（在50 nM酮色林存在的情况下）标记D2和D1 DA受体。DA受体亚型呈现出一种拓扑学上不重叠的分布，总体上与杏仁核复合体各组成核团及亚核区的细胞构筑边界相符。在主要的插入细胞群和中央杏仁核中观察到[125I]碘舒必利结合位点的密度最高，其结合位点从内侧到外侧的梯度定位于其亚核区。[125I]SCH 23982结合位点定位于主要的插入细胞群和基底外侧杏仁核，在中央核中密度均匀较低。因此，中杏仁核DA神经元的功能拓扑结构可能部分在DA受体亚型水平上介导。[125I]碘舒必利结合位点在中央杏仁核亚区及终纹床核中的分布模式表明，“扩展杏仁核”（基底前脑功能组织的一个主要系统）的功能可能在D2受体作用的多个关键位点受到DA传入纤维的调节。
