Blasig J, Papeschi R, Gramsch C, Herz A
Drug Alcohol Depend. 1976 Feb;1(3):221-39. doi: 10.1016/0376-8716(76)90031-4.
The effects of different manipulations of brain serotonin (5-HT) content on the development of morphine dependence were investigated in rats, which were implanted with morphine pellets for 40 days. Serotonin content was decreased by (a) short or long term inhibition of tryptophan hydroxylase with para-chlorophenylalanine (PCPA), (b) by short or long term degeneration of 5-HT containing nerve terminals with 5,6-dihydroxytryptamine or (c) by degeneration of 5-HT containing nerve terminals by lesioning of midbrain raphe nuclei. With all methods used, the frequency of withdrawal jumping was significantly reduced, while other withdrawal signs remained more or less unchanged. Additional administration of 5-HTP to chronically PCPA treated rats did not reverse the PCPA effect. Since chronic reduction of 5-HT level during the whole time of morphine exposure changed withdrawal symptomatology in nearly the same way as did a decrease in 5-HT level during the time of withdrawal only, it is suggested that serotonergic mechanisms are not linked to the basic processes underlying dependence development but that they are only involved in the nervous pathways mediating the expression of some withdrawal signs.
在植入吗啡丸40天的大鼠中，研究了不同方式调控脑内血清素（5-羟色胺，5-HT）含量对吗啡依赖形成的影响。通过以下方式降低血清素含量：（a）用对氯苯丙氨酸（PCPA）短期或长期抑制色氨酸羟化酶；（b）用5,6-二羟基色胺短期或长期使含5-HT的神经末梢变性；（c）通过损伤中脑缝核使含5-HT的神经末梢变性。使用的所有方法均使戒断跳跃频率显著降低，而其他戒断症状或多或少保持不变。对长期接受PCPA治疗的大鼠额外给予5-羟色氨酸（5-HTP）并未逆转PCPA的作用。由于在整个吗啡暴露期间血清素水平的慢性降低与仅在戒断期间血清素水平降低对戒断症状的改变几乎相同，因此提示血清素能机制与依赖形成的基本过程无关，而仅参与介导某些戒断症状表达的神经通路。
