BACHRA B N, TRAUTZ O R, McCONNELL D, FRAJOLA W J, DEAMER D W
Science. 1962 Aug 3;137(3527):337-8. doi: 10.1126/science.137.3527.337.
On theoretical grounds it is unlikely that the catalytic action of the enzyme carbonic anhydrase would be required for the precipitation of apatite in vitro. The presence of carbonic anhydrase in either active or inactivate form did not initiate precipitation in a metastable calcifying solution. It is unlikely that carbonate (or bicarbonate) ions are essential for the precipitation of apatite in vitro or in vivo.
基于理论依据，碳酸酐酶的催化作用在体外磷灰石沉淀过程中不太可能是必需的。无论碳酸酐酶以活性形式还是失活形式存在，都不会在亚稳钙化溶液中引发沉淀。碳酸根（或碳酸氢根）离子在体外或体内磷灰石沉淀过程中不太可能是必需的。
