Bains Amrita, Dhami Navdeep Kaur, Mukherjee Abhijit, Reddy M Sudhakara
Department of Biotechnology, Thapar University, Patiala, 147004, Punjab, India.
Appl Biochem Biotechnol. 2015 Apr;175(7):3531-41. doi: 10.1007/s12010-015-1524-3. Epub 2015 Feb 5.
Microbially induced calcium carbonate precipitation is an immensely growing technology for restoration and remediation of building materials. The investigation on role of exopolymeric substances and biofilms in microbially induced calcium carbonate precipitation suggested that these exopolymeric materials play major role in carbonate precipitation in Bacillus megaterium SS3 along with enzymes urease and carbonic anhydrase. The concentration of EPS directly affects the precipitation of carbonate precipitates which might be due to capturing of Ca(2+) ions by acting as nucleation site. Components of the media and presence of calcium also play crucial role in production of exopolymeric substances along with affecting the morphology of carbonate precipitates.
微生物诱导碳酸钙沉淀是一种在建筑材料修复和整治方面迅速发展的技术。关于胞外聚合物和生物膜在微生物诱导碳酸钙沉淀中作用的研究表明，这些胞外聚合物材料以及脲酶和碳酸酐酶在巨大芽孢杆菌SS3的碳酸盐沉淀中起主要作用。胞外聚合物的浓度直接影响碳酸盐沉淀物的沉淀，这可能是由于其作为成核位点捕获Ca(2+)离子所致。培养基的成分和钙的存在在胞外聚合物的产生中也起着关键作用，同时还会影响碳酸盐沉淀物的形态。
