来自组织细胞培养物及人类生殖器部位的类胸膜肺炎微生物的抗原特性。
Antigenic properties of pleuropneumonia-like organisms from tissue cell cultures and the human genital area.
作者信息
Bailey J S, Clark H W, Felts W R, Fowler R C, Brown T M
出版信息
J Bacteriol. 1961 Oct;82(4):542-7. doi: 10.1128/jb.82.4.542-547.1961.
Bailey, Jack S. (George Washington University, Washington, D. C.), Harold W. Clark, William R. Felts, Richard C. Fowler, and Thomas McP. Brown. Antigenic properties of pleuropneumonia-like organisms from tissue cell cultures and the human genital area. J. Bacteriol. 82:542-547. 1961.-Antigens were prepared from several tissue culture and human genital strains of pleuropneumonia-like organisms (PPLO) by a method utilizing continuous agitation of the incubating cultures. Antisera were produced in rabbits by intravenous injection of suspensions of these organisms standardized turbidimetrically. The antigenic properties of the selected strains were compared by agglutination techniques supplemented by a test based upon the inhibition of growth of PPLO by specific antisera.The majority of tissue culture strains of PPLO studied, including contaminants from several HeLa cell lines, appeared to be antigenically similar to the human type 1 strains. However, one strain (Sp-1) from a HeLa cell line was found to be related to the human type 2 PPLO.
贝利，杰克·S.（乔治·华盛顿大学，华盛顿特区），哈罗德·W.克拉克，威廉·R.费尔茨，理查德·C.福勒，以及托马斯·麦克P.布朗。来自组织细胞培养物和人类生殖器部位的类胸膜肺炎微生物的抗原特性。《细菌学杂志》82:542 - 547。1961年。——通过对培养中的培养物持续搅拌的方法，从几种组织培养和人类生殖器类胸膜肺炎微生物（PPLO）菌株中制备抗原。通过静脉注射经比浊法标准化的这些微生物悬液，在兔体内产生抗血清。通过凝集技术并辅以基于特异性抗血清对PPLO生长抑制的试验，比较所选菌株的抗原特性。所研究的大多数PPLO组织培养菌株，包括来自几种海拉细胞系的污染物，在抗原性上似乎与人类1型菌株相似。然而，发现一株来自海拉细胞系的菌株（Sp - 1）与人类2型PPLO相关。
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