Becker W, Horneff G, Emmrich F, Burmester G, Kalden J, Wolf F
Department of Nuclear Medicine, University of Erlangen-Nürnberg, FRG.
Nuklearmedizin. 1992 Jun;31(3):84-90.
The availability of radiolabeled anti-CD4 antibodies permitted the study of their kinetic behaviour. Four patients suffering from rheumatoid arthritis were investigated prospectively. Three of them received 250 micrograms of 99mTc-labelled anti-CD4 antibody (MAX.16H5). One patient received in vitro 99mTc-antibody-labelled lymphocytes. 4% of the activity was excreted by the kidneys. From 4 to 24 h the splenic uptake decreased from 7.5 to 4%, the liver uptake increased from 25 to 30% the bone marrow uptake remained about the same 50% and the uptake of a single diseased joint (2%) increased slightly to 2.5%. 15 to 30 min after injection of the antibody a redistribution of labelled cells or antibody from liver and spleen to the circulating blood seemed to occur. The recovery rate (0-1 h) of in vivo labelled cells amounted to 30%, that of in vitro labelled cells to 19%. One patient was examined with in vitro labelled CD4-expressing lymphocytes. There was no difference in the antibody kinetics between in vitro and in vivo labelled cells. The authors suggest that circulating CD4-lymphocytes can be labelled with monoclonal antibodies. The kinetics of these in vitro labelled cells and the injected labelled antibody itself resembles that of recirculating lymphocytes.
放射性标记抗CD4抗体的可得性使得对其动力学行为的研究成为可能。对4名类风湿性关节炎患者进行了前瞻性研究。其中3名患者接受了250微克的99mTc标记抗CD4抗体（MAX.16H5）。1名患者接受了体外99mTc抗体标记的淋巴细胞。4%的活性通过肾脏排泄。在4至24小时内，脾脏摄取率从7.5%降至4%，肝脏摄取率从25%升至30%，骨髓摄取率保持在约50%不变，单个患病关节的摄取率（2%）略有增加至2.5%。注射抗体后15至30分钟，标记细胞或抗体似乎从肝脏和脾脏重新分布到循环血液中。体内标记细胞的回收率（0至1小时）为30%，体外标记细胞的回收率为19%。对1名患者进行了体外标记表达CD4淋巴细胞的检查。体外和体内标记细胞之间的抗体动力学没有差异。作者认为循环CD4淋巴细胞可用单克隆抗体进行标记。这些体外标记细胞和注射的标记抗体本身的动力学类似于再循环淋巴细胞的动力学。
