Becker W, Emmrich F, Horneff G, Burmester G, Seiler F, Schwarz A, Kalden J, Wolf F
Department of Nuclear Medicine, Clinical Research Group for Rheumatology/Immunology, Frankfurt, Federal Republic of Germany.
Eur J Nucl Med. 1990;17(3-4):156-9. doi: 10.1007/BF00811445.
CD4 expressing T-lymphocytes are involved in the pathogenesis of rheumatoid arthritis, so the possibility of using radiolabelled CD4-specific antibodies to localise diseased joints was studied. Prospectively six patients with rheumatoid arthritis were investigated. Five of them received 200-300 micrograms of a 555 MBq technetium 99m CD4-specific antibody (MAX.16H5) and were examined with three phase bone scans. Max.16H5 (IgG1) was labelled according to the mercaptoethanol (Schwarz) method. Lymphocytes of one patient were isolated on a Ficoll-Hypaque gradient and labelled with the antibody in vitro. Scans were performed 1.5 h, 4 and 24 h post injection in anterior and posterior views. In all patients, diseased joints could be clearly imaged at as early as 1.5 h. The localisation of the diseased joints correlated (P less than 0.01) with the clinical signs, with the early methylene diphosphonate (MDP) scan (P less than 0.01) and only weakly with the late bone scan (P greater than 0.05). According to these data we conclude that 99mTc-labelled CD4-specific antibodies specifically image actively diseased joints in rheumatoid arthritis.
表达CD4的T淋巴细胞参与类风湿性关节炎的发病机制，因此研究了使用放射性标记的CD4特异性抗体定位患病关节的可能性。前瞻性地对6例类风湿性关节炎患者进行了研究。其中5例接受了200 - 300微克含555兆贝克勒尔锝99m的CD4特异性抗体（MAX.16H5），并进行了三相骨扫描。MAX.16H5（IgG1）根据巯基乙醇（施瓦茨）方法进行标记。对1例患者的淋巴细胞在Ficoll - Hypaque梯度上进行分离，并在体外与抗体进行标记。在注射后1.5小时、4小时和24小时进行前后位扫描。在所有患者中，早在1.5小时就能清晰地对患病关节进行成像。患病关节的定位与临床体征相关（P < 0.01），与早期亚甲基二膦酸盐（MDP）扫描相关（P < 0.01），而与晚期骨扫描相关性较弱（P > 0.05）。根据这些数据我们得出结论，锝99m标记的CD4特异性抗体可特异性地对类风湿性关节炎中活动的患病关节进行成像。
