Thompson N E, Pattee P A
J Bacteriol. 1977 Feb;129(2):778-88. doi: 10.1128/jb.129.2.778-788.1977.
When used in a helper phage capacity, phages 29, 52, 52A, 79, 80, 55, 71, 53, 83A, 85, 95, 96, phi11, and 80 alpha, all serological group B Staphylococcus phages, conferred competence for transformation to S. aureus 8325-4, a strain that does not normally become competent. Of the serological group A phages tested, only phage 3A showed significant competence-conferring activity. Phages 29, 55, 53, 83A, .85, 95, phi11, and 80 alpha showed an enhancement of competence-conferring activity if exposure to the cells occurred in the presence of nromal rabbit serum. All of the propagating strains for the Staphylococcus reference typing phages were rendered competent for transformation by exposure to at least one of these helper phages. The use of a helper phage to confer competence to S. aureus did not result in distortion of the genetic linkages observed in an inherently competent strain. Lysogenization by phages phi11 or 83A is shown not to be required for the expression of competence, and evidence is presented which indicates that competence in the inherently competent 8325 strain is due to a helper phage effect initiated by the adsorption to cells of phi11 virion parts [or phi11 particles in the case of the single lysogen 8325-4(phi11)] that have been liberated by prophage induction.
当以辅助噬菌体的能力使用时，噬菌体29、52、52A、79、80、55、71、53、83A、85、95、96、phi11和80α，所有血清学B组葡萄球菌噬菌体，都能使金黄色葡萄球菌8325 - 4获得转化能力，该菌株通常不会获得感受态。在所测试的血清学A组噬菌体中，只有噬菌体3A显示出显著的赋予感受态的活性。如果在正常兔血清存在的情况下使细胞接触噬菌体29、55、53、83A、85、95、phi11和80α，它们赋予感受态的活性会增强。所有用于葡萄球菌参考分型噬菌体的增殖菌株通过接触至少一种这些辅助噬菌体而获得转化能力。使用辅助噬菌体赋予金黄色葡萄球菌感受态不会导致在固有感受态菌株中观察到的遗传连锁发生畸变。研究表明，噬菌体phi11或83A的溶原化对于感受态的表达不是必需的，并且有证据表明，固有感受态的8325菌株中的感受态是由于前噬菌体诱导释放的phi11病毒粒子部分[对于单溶原菌8325 - 4(phi11)则是phi11颗粒]吸附到细胞上引发的辅助噬菌体效应。
