Wong N L, Wong E F
Department of Medicine, University of British Columbia, Vancouver, Canada.
Nephron. 1992;61(4):464-9. doi: 10.1159/000186967.
Studies were done in partially nephrectomized rats to examine the effect of dietary sodium intake on atrial natriuretic factor (ANF) released by the atria. Experiments were done in four groups of male Wistar rats. Group 1 (n = 10) and 3 (m = 10) rats were sham-operated. Group 2 and 4 were 5/6 nephrectomized. Group 1 and 2 were fed a sodium-supplemented diet. Group 3 and 4 received a sodium-deficient diet. Renal functions were similar between group 2 and 4. Plasma ANF level was raised in group 2 (182 +/- 17 pg/ml). Circulating ANF levels in group 1,3 and 4 were 95 +/- 5, 90 +/- 5 and 95 +/- 4 pg/ml, respectively. Atrial ANF contents were higher in partially nephrectomized rats after receiving a sodium-supplemented diet. A reduction in atrial ANF contents occurred when fed a sodium-deficient diet. In vitro studies were done to assess the rate of ANF released. ANF secretory rates were highest in group 2 (11 +/- 1.5 pg/min/mg). There was no difference between group 1,3 and 4. A positive correlation was found between plasma ANF and ANF released in all groups examined. Thus, plasma ANF levels were a good reflection of ANF secretory rates. A significant correlation existed between plasma ANF and sodium excretion in chronic renal failure rats (r = 0.78; p less than 0.01). A dissociation between plasma ANF and water excretion was seen. These results suggest that in chronic renal failure rats, ANF played a role in sodium adaptation.
为研究饮食中钠摄入量对心房利钠因子（ANF）释放的影响，对部分肾切除大鼠进行了相关研究。实验选用四组雄性Wistar大鼠。第1组（n = 10）和第3组（m = 10）大鼠接受假手术。第2组和第4组大鼠进行5/6肾切除。第1组和第2组大鼠喂食高钠饮食。第3组和第4组大鼠喂食低钠饮食。第2组和第4组大鼠的肾功能相似。第2组大鼠的血浆ANF水平升高（182±17 pg/ml）。第1组、第3组和第4组大鼠的循环ANF水平分别为95±5、90±5和95±4 pg/ml。接受高钠饮食的部分肾切除大鼠心房ANF含量较高。喂食低钠饮食时，心房ANF含量降低。进行体外研究以评估ANF的释放速率。第2组大鼠的ANF分泌速率最高（11±1.5 pg/min/mg）。第1组、第3组和第4组之间无差异。在所检测的所有组中，血浆ANF与释放的ANF之间呈正相关。因此，血浆ANF水平很好地反映了ANF分泌速率。慢性肾衰竭大鼠的血浆ANF与钠排泄之间存在显著相关性（r = 0.78；p < 0.01）。观察到血浆ANF与水排泄之间存在分离。这些结果表明，在慢性肾衰竭大鼠中，ANF在钠适应性调节中发挥作用。
